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Permanent link (DOI): https://doi.org/10.7939/R3SF2MP2M

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The Expression of Transmembrane AMPA Receptor Regulatory Proteins (TARPs) in Developing Zebrafish (Danio rerio) Open Access

Descriptions

Other title
Subject/Keyword
Zebrafish
AMPA Receptors
TARPs
AMPA
AMPAR
Stargazin
Developmental
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Cunningham, Marcus E
Supervisor and department
Ali, Declan (Biological Sciences)
Examining committee member and department
Greer, John (Physiology)
McKenzie, Debbie (Biological Sciences)
Stafford, James (Biological Sciences)
Department
Department of Biological Sciences
Specialization
Physiology, Cell and Developmental Biology
Date accepted
2013-09-16T13:38:43Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
In embryonic vertebrates, newly-formed synapses are quiet until they are unsilenced through a process that resembles synaptic plasticity but remains mechanistically unclear. I used gene expression to determine if Transmembrane AMPA Receptor Regulatory Proteins (TARPs), AMPA receptor auxiliary subunits critical for synaptic plasticity in adults, could be involved in creating the excitability of neurons during development. The sequenced genome of zebrafish makes measuring and manipulating their genetic expression relatively simple, and their quick development into free-swimming larva makes behavioural studies after antisense gene knockdown possible. The duplicated TARP genes of zebrafish are orthologous to those of mice, and were found to be expressed in two waves starting at 12-36hpf or 48hpf. The developmentally important γ2 and γ4 isoforms are expressed ubiquitously at 12hpf, but are exclusive to the nervous tissue of the head by 48hpf. Antisense knockdown experiments failed to show a phenotype distinguishable from that of off-target effects.
Language
English
DOI
doi:10.7939/R3SF2MP2M
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File format: pdf (Portable Document Format)
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File size: 5959719
Last modified: 2015:10:12 20:44:54-06:00
Filename: Cunningham_Marcus_Fall 2013.pdf
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File author: Marcus Cunningham
Page count: 140
File language: en-GB
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