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Permanent link (DOI): https://doi.org/10.7939/R3HX16015

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Effects of drought on Eleusine coracana (L.) Gaertn. (finger millet) and identification of microsatellite markers Open Access

Descriptions

Other title
Subject/Keyword
Drought
SSR Markers
Finger Millet
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Ramadoss, Anupreeti
Supervisor and department
Deyholos, Michael (Department of Biological Science)
Kav, Nat (Department of Agriculture Food and Nutritional Science)
Examining committee member and department
Deyholos, Michael (Department of Biological Science)
Spaner, Dean (Department of Agriculture Food and Nutritional Science)
Kav, Nat (Department of Agriculture Food and Nutritional Science)
Department
Department of Biological Sciences
Specialization
Plant Biology
Date accepted
2014-01-14T13:07:04Z
Graduation date
2014-06
Degree
Master of Science
Degree level
Master's
Abstract
Finger millet (Eleusine coracana (L.) Gaertn.) is a crop with high nutritional profile cultivated mainly in developing countries. It is an allotetraploid (2n= 4x= 36 chromosomes), that belongs to the Poaceae family and has a genome size of 2509 Mbp. Drought is one amongst other abiotic stresses limiting a plant’s growth and productivity. In order to better understand the physiological responses of finger millet to drought, 12 parameters were studied by subjecting plants to water withholding at 26 DAS and imparting water stress for 6 days. Analyzing control plants in parallel indicated that IE 7079 was the most sensitive accession and IE 5091 was the most tolerant accession to water withholding. 83 accessions of finger millet obtained from ICRISAT was genotyped using 31 polymorphic simple sequence repeats (SSRs) identified from transcript assemblies. A total of 152 alleles were generated by these 31 SSRs, with a mean of 4.9 alleles per locus and mean polymorphism information content (PIC) of 0.49 Cluster analysis of these accessions showed considerable genetic variation in the varieties from different geographical origins. Identified polymorphic SSRs can be used to further expand the linkage map of finger millet.
Language
English
DOI
doi:10.7939/R3HX16015
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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