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Permanent link (DOI): https://doi.org/10.7939/R3HT2GK99

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The Effect of Interleukin 25 on Human Th2 Lymphocytes Open Access

Descriptions

Other title
Subject/Keyword
Interleukin 25
Th2
Allergy
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Bredo, Graeme, K
Supervisor and department
Cameron, Lisa (Medicine)
Examining committee member and department
Baldwin, Troy (Medical Microbiology and Immunology)
Barreda, Daniel (Biological Sciences)
Vliagoftis, Harissos (Medicine)
Department
Department of Medicine
Specialization
Experimental Medicine
Date accepted
2014-01-30T11:56:06Z
Graduation date
2014-06
Degree
Master of Science
Degree level
Master's
Abstract
CRTh2 (chemoattractant receptor-homologous molecule expressed on Th2 cells) is a marker for Th2 cells and activation through CRTh2 stimulates expression of cytokines that are important for allergic responses such as IL-4, IL-5 and IL-13. We have observed CRTh2+ T cells express the IL-25 receptor (IL-25R). IL-25 is produced by the epithelium in response to allergens, parasites, and viruses. We hypothesized IL-25 would modulate acquisition of the Th2 phenotype. The effect of IL-25 on Th2 differentiation was investigated by culturing naive human CD4 T cells in the presence or absence of IL-25. IL-25 alone induced expression of IL-4, GATA3 and CRTh2. Head to head comparison with the canonical Th2 cytokine, IL-4, showed that IL-25 was equally effective in inducing IL-4 expression. These findings suggest IL-25 initiates Th2 differentiation and amplifies production of Th2 cytokines. Consequently, IL-25 inducing pathogens such as RSV may play a role in initiating and exacerbating allergic diseases.
Language
English
DOI
doi:10.7939/R3HT2GK99
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File title: The Influence of IL-25 on Human Th2 Lymphocytes
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Page count: 125
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