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Structure and Stability of Microbial Assemblages in Seasonal Lake Ice: Miquelon Lake, Alberta, Canada Open Access


Other title
Miquelon Lake Alberta
microbial composition
seasonal lake ice
Type of item
Degree grantor
University of Alberta
Author or creator
Bramucci, Anna
Supervisor and department
Lanoil, Brian (Biological Sciences)
Examining committee member and department
Haas, Christian (Earth and Atmospheric Sciences)
Case, Rebecca (Biological Sciences)
Department of Biological Sciences
Microbiology and Biotechnology
Date accepted
Graduation date
Master of Science
Degree level
Prokaryotic life has been identified in every terrestrial icy/frozen environment studied to date. There is evidence of microbial activity and replication within ice as well, but there has been little research done on the seasonally frozen portion of the cryosphere. The present study focuses on the inter- and intra-seasonal microbial assemblage composition within the lake ice of Miquelon Lake, a shallow saline lake in Alberta, Canada. The prokaryotic diversity was investigated by screening diversity with denaturing gradient gel electrophoresis (DGGE) and subsequently constructing bacterial and eukaryotic clone libraries of the 16S and 18S rRNA genes, respectively. The nearest neighbors (according to BLAST) indicate the presence of a complete microbial food web within the lake ice throughout the duration of the winter season. Furthermore, the presence of a chlorophyll a peak at a conserved ice depth throughout the duration of the season gives indication that at lease a portion of the photosynthetic ice consortia might be active or reproducing within the ice.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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