Download the full-sized PDF
Permanent link (DOI): https://doi.org/10.7939/R3WP9TB2M
This file is in the following communities:
|Biological Sciences, Department of|
This file is in the following collections:
|Journal Articles (Biological Sciences)|
The procambium specification gene Oshox1 promotes polar auxin transport capacity and reduces its sensitivity toward inhibition Open Access
- Author or creator
Boot, K. J. M.
Meijer, A. H.
- Additional contributors
Tracheary Element Differentiation
- Type of item
- Journal Article (Published)
The auxin-inducible homeobox gene Oshox1 of rice (Oryza sativa) is a positive regulator of procambial cell fate commitment, and its overexpression reduces the sensitivity of polar auxin transport (PAT) to the PAT inhibitor 1-N-naphthylphthalamic acid (NPA). Here, we show that wild-type rice leaves formed under conditions of PAT inhibition display vein hypertrophy, reduced distance between longitudinal veins, and increased distance between transverse veins, providing experimental evidence for a role of PAT in vascular patterning in a monocot species. Furthermore, we show that Oshox1 overexpression confers insensitivity to these PAT inhibitor-induced vascular-patterning defects. Finally, we show that in the absence of any overt phenotypical change, Oshox1 overexpression specifically reduces the affinity of the NPA-binding protein toward NPA and enhances PAT and its sensitivity toward auxin. These results are consistent with the hypothesis that Oshox1 promotes fate commitment of procambial cells by increasing their auxin conductivity properties and stabilizing this state against modulations of PAT by an endogenous NPA-like molecule.
- Date created
- License information
- © 2002 American Society of Plant Biologists. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
- Citation for previous publication
Scarpella, E., Boot, K. J. M., Rueb, S., & Meijer, A. H. (2002). The procambium specification gene Oshox1 promotes polar auxin transport capacity and reduces its sensitivity toward inhibition. Plant Physiology, 130(3), 1349-1360. doi: 10.1104/pp.009167.
- Link to related item
- Date Uploaded
- Date Modified
- Audit Status
- Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 389401
Last modified: 2015:10:12 18:19:21-06:00
Original checksum: 59f30db6395b372f922ff57eca5a0577
Well formed: true
Page count: 12