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Permanent link (DOI): https://doi.org/10.7939/R3CW7W

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Theses and Dissertations

Novel polymer and lipid-based nanocarriers for gene delivery Open Access

Descriptions

Other title
Subject/Keyword
gene delivery
polymers
liposomes
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Fitzsimmons, Ross
Supervisor and department
Uludag, Hasan (Chemical and Materials Engineering)
Examining committee member and department
Unsworth, Larry (Chemical and Materials Engineering)
Narain, Ravin (Chemical and Materials Engineering)
Department
Department of Biomedical Engineering
Specialization

Date accepted
2011-11-17T21:51:58Z
Graduation date
2012-06
Degree
Master of Science
Degree level
Master's
Abstract
The following thesis describes original studies assessing the gene delivery efficacy of novel non-viral carrier combinations. The first panel of non-viral carriers tested are termed AVPs (artificial viral particles), in reference to their structural similarity to enveloped viruses, and were fabricated in a composite manner using a variety of polymers and lipids in order to deliver plasmid DNA. The most effective carrier found in these studies was 25 kDa PEI (polyethylenimine), with none of the AVPs showing a clear superior effect to that of 25 kDa PEI. While a highly effective gene carrier, PEI is extremely cytotoxic and hence PEI was conjugated with PEG (polyethylene glycol) in attempt to decrease its toxicity while maintaining its functionality. Ultimately, it was found that PEGylation generally decreased the transfection efficacy of PEI, but under ideal conditions of PEG substitution and polymer/DNA (w/w) ratio, this decrease in efficacy can be circumvented.
Language
English
DOI
doi:10.7939/R3CW7W
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File title: RossFitzsimmonsMScThesis Nov 2 PC before scope.pdf
File author: Ross Fitzsimmons
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