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Permanent link (DOI): https://doi.org/10.7939/R3JH3DB0K

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The function of nonmuscle myosins during oogenesis in Caenorhabditis elegans Open Access

Descriptions

Other title
Subject/Keyword
MLC-4
Caenorhabditis elegans
NMY-1
nonmuscle myosins
Oogenesis
NMY-2
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Pisio, Amanda C
Supervisor and department
Pilgrim, Dave (Biological Sciences)
Examining committee member and department
Hitt, Mary (Oncology)
King-Jones, Kirst (Biological Sciences)
Srayko, Martin (Biological Sciences)
Department
Department of Biological Sciences
Specialization
Molecular Biology and Genetics
Date accepted
2013-10-02T14:06:08Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
Oogenesis in C. elegans requires the activity of type II nonmuscle myosins, complexes containing two heavy and four light chains that bind and move actin. Two different complexes are found in the gonad, differing in their heavy chains, (NMY-1 versus NMY-2). These two myosins are redundant during embryonic elongation, and we expected them to also be redundant during oogenesis. Instead, both myosins are required, but their requirement differs at different stages. NMY-2 is required for maintenance and then closure of the ring channels, while both NMY-1 and NMY-2 are required for cytoplasmic streaming. Finally, NMY-1 is required for ovulation. Loss of MLC-4, the regulatory light chain, did not reveal phenotypes other than those seen with the loss of either myosin, meaning that the myosins do not act redundantly during oogenesis. Therefore, the different heavy chains apparently allows differential regulation of separate functions during this process.
Language
English
DOI
doi:10.7939/R3JH3DB0K
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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