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Delivery of siRNA using Lysine-Functionalized Rosette Nanotubes for Cancer Therapy Open Access


Other title
Protein silencing
Cancer therapy
Rosette Nanotubes
siRNA delivery
Type of item
Degree grantor
University of Alberta
Author or creator
Ho, Uyen T
Supervisor and department
Fenniri, Hicham (Chemistry)
Examining committee member and department
Vederas, John (Chemistry)
Campbell, Robert (Chemistry)
Department of Chemistry

Date accepted
Graduation date
Master of Science
Degree level
Cancer remains to be one of the deadliest diseases world-wide due to its effect on millions of lives per year. The discovery of RNA interference and delivery of siRNA (small-interfering RNA) for protein silencing have been widely used for treatment of cancer cells. Research in our lab focuses on G∧C molecules (hybrid of guanine and cytosine bases) that can self-assemble into rosette nanotubes (RNTs) in physiological environment, proves to be biocompatible for in vitro and in vivo applications. This dissertation explores the lysine functionalized-twin RNTs (KnT RNTs, n = number of lysine residues) and lysine-functionalized mono RNTs (K1 RNTs). Gel retardation assay and fluorescence imaging showed that cationic charges on the RNTs strongly affect the binding interaction with siRNA and the intracellular delivery of the RNTs-siRNA complexes. K3T RNTs and K1 RNTs were assessed to demonstrate their low toxicity and effectiveness in siRNA delivery for protein silencing in cancer cells (A549 and HCT116).
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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