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Permanent link (DOI): https://doi.org/10.7939/R3536Q

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BAD-interacting proteins in breast cancer cells Open Access

Descriptions

Other title
Subject/Keyword
BCL-XL
BCL-2 antagonist of cell death
apoptosis
BAD
14-3-3
paclitaxel
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Craik, Alison C
Supervisor and department
Goping, Ing Swie (Biochemistry)
Examining committee member and department
Bleackley, Christopher (Biochemistry)
Simmen, Thomas (Cell Biology)
Fahlman, Richard (Biochemistry)
Department
Department of Biochemistry
Specialization

Date accepted
2012-05-16T16:02:02Z
Graduation date
2012-11
Degree
Master of Science
Degree level
Master's
Abstract
Taxane chemotherapy is widely used in the treatment of breast cancer. Despite its widespread clinical use, the molecular mechanisms of paclitaxel induced apoptosis remain unclear. Our laboratory has identified an important role for the BH3-only protein, BAD, in the paclitaxel induced cell death pathway of breast cancer cells. Furthermore, BAD expression correlates with sensitivity to taxanes in vitro and in clinical studies. While BAD is a well characterized pro-apoptotic member of the BCL-2 family, our studies demonstrate a novel, non-apoptotic role for BAD in paclitaxel-induced cell death. Specifically, this work suggests a role for BAD in cell cycle progression or diminished apoptotic signaling, consistent with data from the laboratory documenting pro-growth activity of BAD. We screened for BAD interacting proteins to improve our understanding of BAD molecular dynamics and identify protein partners that may contribute to BAD pro-growth activity. BAD interactions with BCL-XL and 14-3-3 protein isoforms were examined.
Language
English
DOI
doi:10.7939/R3536Q
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Craik AC, Veldhoen RA, Czernick M, Buckland TW, Kyselytzia K, Ghosh S, et al. The BH3-only protein Bad confers breast cancer taxane sensitivity through a nonapoptotic mechanism. Oncogene 2010 Sep 30;29(39):5381-5391.

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