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Linkage mapping, phenotypic characterization, and introgression analysis of crosses with safflower (Carthamus tinctorius) Open Access


Other title
Linkage mapping, introgression, safflower, Carthamus tinctorius, Carthamus oxyacanthus, oilseed
Type of item
Degree grantor
University of Alberta
Author or creator
Archibald, Catherine J.
Supervisor and department
Good, Allen (Biological Sciences)
Examining committee member and department
Spaner, Dean (Agriculture, Food and Nutritional Sciences)
Coltman, David (Biological Sciences)
Cahill, James C. (Biological Sciences)
Department of Biological Sciences

Date accepted
Graduation date
Master of Science
Degree level
An inter-specific linkage map of a backcross (BC1) population derived from a cross between C. tinctorius and C. oxyacanthus was developed using primarily microsatellite markers. A composite map, which aligned the inter-specific (C. tinctorius x C. oxyacanthus) map with an intra-specific linkage map previously developed from an F2 population of C. tinctorius, was created to search for syntenic regions. Results indicate that despite low marker saturation, there is substantial colinearity between the two linkage maps, and one translocation or inversion event. Upon subsequent self-fertilization events, phenotypes of the inter-specific backcross population were characterized in both the field (BC1S2 generation) and growth chamber (BC1S2 and BC1S3 generations), and identified several lines of agronomic interest. Introgression analysis was performed (BC1S3 generation) to assess the level of integration of C. oxyacanthus DNA into the C. tinctorius genome, and results are suggestive of less inter-specific recombination than expected.
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