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Permanent link (DOI): https://doi.org/10.7939/R31X1V

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Evaluation of Derivatization Reaction Conditions for Metabolite Labelling Using Differential Isotopic Labelling for Biological Samples Analyzed by Liquid Chromatography Mass Spectrometry Open Access

Descriptions

Other title
Subject/Keyword
Dansylation
metabolomics
Isotopic Labeling
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Curle, Jared P
Supervisor and department
Li, Liang (Chemistry)
Examining committee member and department
Li, Xing-Fang (Laboratory Medicine and Pathology)
Lucy, Charles (Chemistry)
Department
Department of Chemistry
Specialization

Date accepted
2013-09-28T21:42:38Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
The objective of this work was to assess the impact of reaction conditions for isotopic labeling of metabolites prior to analysis using liquid chromatography mass spectrometry (LC-MS). Differential isotope 12C/13C dansyl chloride was used to label metabolites containing either primary amines, secondary amines, or phenolic functional groups and those were determined by both liquid chromatography electrospray ionization Fourier transform ion cyclotron resonance MS (LC-ESI-FTICR-MS) and ultra-performance liquid chromatography with ultraviolet absorbance detection (UPLC-UV). Using UPLC-UV, metabolite standards labeled under varying reaction conditions were quantitatively determined for comparison. Compared with conventional heating, microwave assisted heating (MAH) methods demonstrated better labeled product yields. In addition ambient temperature reactions were found provide the best labelling without using MAH. It was found that a reaction temperature of 40°C provided the best results based on the number of peak pairs found. However other reaction methods provided unique peak pairs not found in the other methods.
Language
English
DOI
doi:10.7939/R31X1V
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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