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Theses and Dissertations

Population genomics of North American grey wolves (Canis lupus) Open Access

Descriptions

Other title
Subject/Keyword
genomics
wolves
SNPs
structure
population
selection
microarray
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Knowles, James
Supervisor and department
Coltman, David (Biological Sciences)
Examining committee member and department
Hall, Jocelyn (Biological Sciences)
Moore, Stephen (Agricultural, Food and Nutritional Science)
Department
Department of Biological Sciences
Specialization

Date accepted
2010-07-26T21:38:20Z
Graduation date
2010-11
Degree
Master of Science
Degree level
Master's
Abstract
Previous studies of the grey wolf (Canis lupus) using microsatellites have showed strong population structure despite the high mobility of individuals. I re-assessed the structure of North American grey wolves by genotyping 132 wolves at a genome-wide set of >26 000 single nucleotide polymorphisms (SNPs), and found less population structure, a strong pattern of isolation by distance, and determined that gene flow between subpopulations relates to prey specialization. To assess how accurately smaller data sets assign individuals, I analyzed sub-sets of SNPs and found that small marker sets varied greatly in estimates of subpopulation assignment, and showed high discordance with assignments determined when using all 26k markers. Finally, using a genome scan to detect natural selection I identified SNPs in three genes that may have undergone directional selection, contain variation with observed phenotypic consequences in other mammal species and may be related to adaptation in grey wolves.
Language
English
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 2153751
Last modified: 2015:10:12 19:50:16-06:00
Filename: Knowles MSc final revisions small.pdf
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Status message: File header gives version as 1.4, but catalog dictionary gives version as 1.3
File title: Microsoft Word - Knowles MSc final revisions.docx
File author: James C Knowles
Page count: 100
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