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Permanent link (DOI): https://doi.org/10.7939/R3GW7T

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Application of genomics-based tools leading to the identification of markers on bovine chromosome 14 influencing milk production and carcass quality traits Open Access

Descriptions

Other title
Subject/Keyword
disequilibrium
production
markers
radiation
genetics
linkage
genomics
association
milk
meat
cattle
improvement
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Ferreira Marques, Elisa
Supervisor and department
Moore, Stephen
Examining committee member and department
Rocha, Dominique
Wang, Zhiquan
Walter, Michael
Dixon, Walter
Department
Department of Agricultural, Food and Nutritional Science
Specialization

Date accepted
2009-07-31T15:00:49Z
Graduation date
2009-11
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
Genetic improvements in beef and dairy cattle can bring significant advances in satisfying the global food demand, which is expected to double by 2050. Several DNA markers have been identified on bovine chromosome 14 (BTA14), but low mapping resolution prevents their refinement for identification of causal mutations. The objective of this research was to apply radiation hybrid mapping technique to correctly map available high density markers, enabling the accurate assessment of linkage disequilibrium and the scanning of quantitative trait loci across the chromosome. The research also applied these techniques to identify candidate markers on BTA14 contributing to the genetic variation observed in milk production and carcass quality traits in Holstein and Angus cattle, respectively. The first study aimed at correctly ordering genetic markers along BTA14 and comparing the order to the bovine sequence assembly to aid collaborative efforts in improving the future versions of the assembly. A 12K radiation hybrid map of BTA14 was constructed using 843 single nucleotide polymorphism markers. The second study assessed the extent of linkage disequilibrium along the chromosome identifying specific regions in both Angus and Holstein cattle where non-random association between alleles of different loci occurred. For both breeds, results showed that average linkage disequilibrium extends to moderate levels up to 100 kilo base pairs and falls to background levels after 500 kilo base pairs. Correlation analysis for marker pairs common to these two breeds confirmed that the same marker phase is maintained only up to distances of 10 kilo base pairs. Linkage analysis studies for both breeds identified markers on the basis of sire heterozygosity and linkage disequilibrium and reported quantitative trait loci affecting milk production and carcass quality traits. Finally, using marker function, association and linkage analysis results, several candidate markers demonstrating significant effects on these economically relevant traits were identified. The results from this study support the existence of considerable genetic variation for both milk production and carcass quality traits in Holstein and Angus cattle, respectively, demonstrating opportunities for genetic improvement.
Language
English
DOI
doi:10.7939/R3GW7T
Rights
License granted by Elisa Ferreira Marques (emarques@ualberta.ca) on 2009-07-24T20:37:42Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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