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Comparison of serum antibody responses to Giardia lamblia of symptomatic and asymptomatic patients Open Access

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Author or creator
Soliman, M.M.
Taghi-Kilani, R.
Abou-Shady, A.F.
El-Mageid, S.A.
Handousa, A.A.
Hegazi, M.M.
Belosevic, M.
Additional contributors
Subject/Keyword
linked immunosorbent-assay
mice
surface gycoprotein
excretory-secretory prodcuts
immunofluorescence test
antigenic variation
muris trophozoites
cross-reactivity
monoclonal-antibodies
infection
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
The circulating anti-parasite antibody response against Giardia lamblia in symptomatic and asymptomatic Egyptian children with confirmed giardiasis was examined. Symptomatic patients were identified using the following criteria: presence of only G. lamblia cysts in the feces, and one or more of the following symptoms, diarrhea, abdominal pain, loss of weight, vomiting and/or nausea, and abdominal distention. The anti-parasite humoral response was measured using indirect immunofluorescence (IFA), ELISA, and immunoblotting. There was a significant difference in the anti-parasite antibody response measured by IFA of asymptomatic and symptomatic patients, in which more than 34% of the asymptomatic patients had a titer equal to or less than 1:500, and more that 29% of the symptomatic patients had a titer of 1:8,000 or higher. The circulating anti-parasite total IgM and IgA but not IgG, measured by ELISA, was significantly higher in symptomatic than in asymptomatic patients, and were related to higher cyst output observed in symptomatic individuals. Although total anti-parasite IgG response was similar in symptomatic and asymptomatic patients, the analysis of the IgG isotype responses revealed that both IgG1 and IgG3 were significantly higher in symptomatic patients. The antigen recognition by anti-parasite IgM, IgA, IgG1, and IgG3 of symptomatic and asymptomatic individuals, determined by immunoblotting, was heterogeneous and revealed only minor differences in the response of the two groups.
Date created
1998
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Copyright 1998 by The American Society...
Citation for previous publication
Soliman, M. M., Taghi-Kilani, R., Abou-Shady, A. F., El-Mageid, S. A., Handousa, A. A., Hegazi, M. M., & Belosevic, M. (1998). Comparison of serum antibody responses to Giardia lamblia of symptomatic and asymptomatic patients. The American Journal of Tropical Medicine and Hygiene, 58(2), 232-239.
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File title: COMPARISON OF SERUM ANTIBODY RESPONSES TO GIARDIA LAMBLIA OF SYMPTOMATIC AND ASYMPTOMATIC PATIENTS
File author: MOHAMED M. SOLIMAN, RUHI TAGHI-KILANI, AHMED F. A. ABOU-SHADY, SALAMA A. A. EL-MAGEID, AYA A. HANDOUSA, MAMDOUH M. HEGAZI, AND MIODRAG BELOSEVIC
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