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Permanent link (DOI): https://doi.org/10.7939/R3X36J

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Scalable, modular, integrated genetic analysis systems Open Access

Descriptions

Other title
Subject/Keyword
polymerase chain reaction
scalable
microfluidics
genetic analysis system
modular
capillary electrophoresis
integrated
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Bidulock, Allison Christel Elizabeth
Supervisor and department
Backhouse, Christopher J. (Electrical and Computer Engineering)
McMullin, James M. (Electrical and Computer Engineering)
Examining committee member and department
Kresta, Suzanne M. (Chemical and Materials Engineering)
Brett, Michael (Electrical and Computer Engineering)
Department
Department of Electrical and Computer Engineering
Specialization

Date accepted
2011-08-16T19:41:07Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
This thesis addresses the need for inexpensive microfluidic platforms by contributing to the collaborative development of a modularised, integrated system capable of molecular diagnostics using bead-based sample preparation, polymerase chain reaction and capillary electrophoresis analysis techniques. Although the entire system is briefly presented here, the primary focus of this project had three central goals: contribution to the system architecture through the sole development of in-house, modular software and firmware-software interface protocol; verification of the system’s capabilities through extensive testing and strict characterisation of its performance; and, the identification of challenges associated with the characterised system and discussion of possible or implemented solutions. With a total component cost of less than $1000, this sytem has optical sensitivity comparable to traditional electrophoretic analysis methods, the ability to amplify from a few molecules, and represents a significant advancement for the use of lab-on-a-chip technologies in point of care applications.
Language
English
DOI
doi:10.7939/R3X36J
Rights
License granted by Allison Bidulock (acb7@ualberta.ca) on 2011-08-11T22:00:29Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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