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Permanent link (DOI): https://doi.org/10.7939/R3P55DP70

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Manganese Porphyrin Protects Neonatal Pig Islets against Oxidative Stress and Enhances their Function in Diabetic Mice Open Access

Descriptions

Other title
Subject/Keyword
Diabetes, Pancreatic Islets, Anti-oxidant
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Nasralla, Awrad
Supervisor and department
Rayat, Gina ( Surgery)
Examining committee member and department
Jahroudi, Nadia (Medicine)
Rajotte, Ray (Surgery)
Korbutt, Gregory (Surgery)
Department
Department of Surgery
Specialization
Experimental Surgery
Date accepted
2015-03-17T11:23:40Z
Graduation date
2015-06
Degree
Master of Science
Degree level
Master's
Abstract
Significant numbers of pancreatic islets are lost during isolation and early transplantation period due to oxidative stress. Neonatal pig islets are being considered to overcome the shortage of human donors. Manganese (III) tetrakis (N-ethylpyridium-2-yl) porphyrin (MnP) is a synthetic antioxidants that was shown to protect human and murine islets from oxidative stress. We report that pre-treatment of neonatal pig islets with MnP resulted in up-regulation of gene expression of antioxidant enzymes such as superoxide dismutase and antiapoptotic molecules such as survivin. We found that MnP is not toxic to neonatal pig islets. Further, MnP provided protection to neonatal pig islets against H2O2 induced damage. In addition, NSG mice that received islets pre-treated with 34 μM MnP achieved normoglycemia earlier and had significantly lower average blood glucose level compared to other groups. We report that MnP could be beneficial to neonatal pig islets provided protection against oxidative stress and enhanced their function in vivo.
Language
English
DOI
doi:10.7939/R3P55DP70
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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