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Permanent link (DOI): https://doi.org/10.7939/R3F18SN1S

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The Sensitivity of Canadian Wheat Genotypes to the Host-Selective Toxins Produced by Pyrenophora tritici-repentis Open Access

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Other title
Subject/Keyword
Host-Selective Toxins, Pyrenophora tritici-repentis,Canadian Wheat Genotypes
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Tran,Van Anh, Thi
Supervisor and department
Strelkov, Stephen (Agricultural, Food and Nutritional Science)
Examining committee member and department
Basu, Urmila (Agricultural, Food and Nutritional Science)
Turkington, Kelly (Agriculture and Agri-Canada Food)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization
Plant Science
Date accepted
2014-07-10T10:56:46Z
Graduation date
2014-11
Degree
Master of Science
Degree level
Master's
Abstract
Tan spot is a foliar disease of wheat caused by the fungus Pyrenophora tritici–repentis. Disease development is associated with the production of three host-selective toxins (Ptr ToxA, Ptr ToxB, and Ptr ToxC) that serve as fungal pathogenicity factors. The aim of this study was to investigate the sensitivity to each of these toxins in a representative collection of 100 Canadian wheat cultivars known as the VarComp population. Heterologously expressed, His-tagged Ptr ToxA and Ptr ToxB, and spore germination fluids possessing Ptr ToxC activity, were produced and used to screen the cultivars for toxin sensitivity. Ninety two of the 100 wheat cultivars tested were sensitive to at least one toxin, with 68% found to be sensitive to Ptr ToxA, 63% sensitive to Ptr ToxC, and 24% sensitive to Ptr ToxB. Eight cultivars were insensitive to all three toxins and may represent an important resource for tan spot resistance breeding activities.
Language
English
DOI
doi:10.7939/R3F18SN1S
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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