Download the full-sized PDF of The secretogranin II-derived peptide secretoneurin stimulates luteinizing hormone secretion from gonadotrophs.Download the full-sized PDF



Permanent link (DOI):


Export to: EndNote  |  Zotero  |  Mendeley


This file is in the following communities:

Biological Sciences, Department of


This file is in the following collections:

Journal Articles (Biological Sciences)

The secretogranin II-derived peptide secretoneurin stimulates luteinizing hormone secretion from gonadotrophs. Open Access


Author or creator
Zhao, E.
Basak, A.
Wong, A.O.L.
Chen, A.
López, G.C.
Grey, C.L.
Canosa, L.F.
Somoza, G.M.
Chang, J.P.
Trudeau, V.L.
Additional contributors
prohormone convertases
neuroendocrine system
goldfish pituitary
Type of item
Journal Article (Published)
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a member of the glucagon/secretin peptide family, and its molecular structure is highly conserved in vertebrates. In this study, the functional role of PACAP in regulating GH release in the goldfish was investigated. Using immunohistochemical staining, nerve fibers with PACAP immunoreactivity were identified in the vicinity of goldfish somatotrophs, suggesting that this neuropeptide may influence GH release in the goldfish. The direct regulatory action of PACAP on GH secretion was demonstrated in vitro in perifused goldfish pituitary cells. PACAPs (0.01 nM to 1mM) from different species, including ovine PACAP27, ovine PACAP38, frog PACAP38, zebra fish PACAP27, and zebra fish PACAP38, were all effective in stimulating GH release with ED50 values of 8.9 6 3.5, 3.3 6 1.6, 14.4 6 3.5, 15.4 6 4.1, and 1.4 6 0.2 nM, respectively. Similar concentrations of vasoactive intestinal polypeptide (VIP), a peptide related to PACAP, was not effective in this respect. In addition, the GH-releasing action of ovine PACAP38 (10 nM) was inhibited by the PACAP antagonist PACAP6–38 (10 mM), but not by the VIP antagonist [4-Cl-D-Phe6,Leu17]VIP (10 mM). The pharmacology of these GH responses is consistent with the mammalian type I PACAP receptors, suggesting that a similar receptor subtype is present in the goldfish pituitary and mediates the GH-releasing action of PACAP. To establish the structural identity of this goldfish PACAP receptor, a complementary DNA (cDNA) clone sharing a high degree of sequence homology with mammalian type I PACAP receptors was isolated from a goldfish pituitary cDNA library. This cDNA was 5.2 kb in size with a 1.4-kb open reading frame and encoded a 465-amino acid protein with the typical structure of a 7-transmembrane domain-containing, G protein-coupled receptor. Functional expression of this cDNA in COS-7 cells revealed that this fish type I PACAP receptor could be activated by ovine PACAP27 and PACAP38 to increase cAMP synthesis with ED50 values of 2.4 6 0.8 and 4.2 6 1.2 nM, respectively. Other structurally related peptides, including VIP (100 nM), GH-releasing hormone (100 nM), glucagon (100 nM), secretin (100 nM), gastric inhibitory polypeptide (100 nM), and PTH (100 nM), were not effective in altering cAMP production. Using Northern blot and RT-PCR, messenger RNA transcripts of this PACAP receptor were identified in the brain, heart, and pituitary of the goldfish. These results, taken together, support the hypothesis that PACAP functions as a novel GH-releasing factor in the goldfish through activation of type I PACAP receptors. (Endocrinology 139: 3465–3479, 1998)
Date created
License information
Copyright © 1998 by The Endocrine Society
Citation for previous publication
Zhao, E., Basak, A., Wong, A.O.L., Ko,W., Chen, A., López, G.C., Grey, C.L., Canosa, L.F., Somoza, G.M., Chang, J.P. and Trudeau, V.L. "The secretogranin II-derived peptide secretoneurin stimulates luteinizing hormone secretion from gonadotrophs." Endocrinology 150 (2009): 2273-2282.
Link to related item

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 744800
Last modified: 2015:10:12 20:33:22-06:00
Filename: Endocrinology_139_1998_3465.pdf
Original checksum: 7b7c06ae1b5c63666671a74ff12d52cb
Well formed: true
Valid: true
Page count: 15
Activity of users you follow
User Activity Date