Download the full-sized PDF of Transcriptomic changes in ruminal tissue induced by the periparturient transition in dairy cowsDownload the full-sized PDF



Permanent link (DOI):


Export to: EndNote  |  Zotero  |  Mendeley


This file is in the following communities:

Agricultural, Food and Nutritional Science, Department of


This file is in the following collections:

Journal Articles (Agricultural, Food and Nutritional Science)

Transcriptomic changes in ruminal tissue induced by the periparturient transition in dairy cows Open Access


Author or creator
Dionissopoulos, Louis
AlZahal, Ousama
Steele, Michael A.
Matthews, James C.
McBride, Brian W.
Additional contributors
Immune System
Fat Metabolism
Transition Period
Biological Interactome
Type of item
Journal Article (Published)
To understand how the capacity for fat metabolism (uptake, synthesis, modification) changes in rumen epithelia immediately before and after onset of lactation in dairy cows, rumen fluid Short Chain Fatty Acid (SCFA) concentrations and mRNA expression profiles of rumen epithelia was determined in twelve Holstein dairy cows at three weeks prior to calving (wk -3, n = 12), one week post calving (week +1, n = 12) and six weeks (week +6, n = 12) after calving. The diet was modified from a dry cow formulation to a lactating cow formulation immediately following parturition and raised the non-fiber carbohydrate level from 34 to 43%. All data was analyzed using the mixed procedure of SAS, with cows blocked by anticipated calving date and week of sampling as the repeated measure. Propionate, butyrate, isovalerate and valerate levels rose significantly following the diet change (p?0.001), although acetate and isobutyrate levels were unchanged (p>0.05). Mean rumen pH also changed during the transition period (6.38 Vs 5.81 and 5.85±0.08; -3 Vs +1 and +6; p<0.001) as did mean BW (716.00 Vs 635.82 kg and 615.45 kg ±16.20; -3 Vs +1 and +6; p?0.002). Microarray analysis of total RNA from rumen epithelial biopsies revealed 1476 differentially expressed genes at a false discovery rate of 10%. These results were filtered for genes that were directly related to both the immune system and fat metabolism/homeostasis. Consequently, the expression of the resulting 28 genes was analyzed by quantitative PCR (qRT-PCR) to compare their expression at period -3 versus +6 periods. qRT-PCR analysis revealed that 13 genes were upregulated (p?0.01), 2 were downregulated (p?0.01) and 13 were unchanged during the transition period. Pathway and context analysis yielded a unique interactome pathway map which revealed a set of genomic interactions that indicate a link between selected genes from the immune system and those involved in the preparation for lactation.
Date created
License information

Attribution 4.0 International
Citation for previous publication
Dionissopoulos, L., AlZahal, O., Steele, M. A., Matthews, J. C., & McBride, B. W. (2014). Transcriptomic changes in ruminal tissue induced by the periparturient transition in dairy cows. American Journal of Animal and Veterinary Sciences, 9(1), 36-45.


Link to related item

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 159306
Last modified: 2017:09:06 16:01:28-06:00
Filename: AJAVS_9_1_36.pdf
Original checksum: be0b154f04b0186d1f3ec1ec6a9fbe36
Well formed: true
Valid: true
File author: User
Page count: 10
Activity of users you follow
User Activity Date