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Permanent link (DOI): https://doi.org/10.7939/R34F1MM6C

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Disentangling detoxification: Gene expression analysis of feeding mountain pine beetle illuminates molecular-level host chemical defense detoxification mechanisms Open Access

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Author or creator
Robert, Jeanne A.
Pitt, Caitlin
Bonnett, Tiffany R.
Yuen, Macaire M. S.
Keeling, Christopher I.
Bohlmann, Jörg
Huber, Dezene P. W.
Additional contributors
Subject/Keyword
Detoxification
Beetles
Gene expression
Invertebrate genomics
cDNA libraries
Genomic libraries
Tribolium
Pines
Type of item
Journal Article (Published)
Language
english
Place
Time
Description
The mountain pine beetle, Dendroctonus ponderosae, is a native species of bark beetle (Coleoptera: Curculionidae) that caused unprecedented damage to the pine forests of British Columbia and other parts of western North America and is currently expanding its range into the boreal forests of central and eastern Canada and the USA. We conducted a large-scale gene expression analysis (RNA-seq) of mountain pine beetle male and female adults either starved or fed in male-female pairs for 24 hours on lodgepole pine host tree tissues. Our aim was to uncover transcripts involved in coniferophagous mountain pine beetle detoxification systems during early host colonization. Transcripts of members from several gene families significantly increased in insects fed on host tissue including: cytochromes P450, glucosyl transferases and glutathione S-transferases, esterases, and one ABC transporter. Other significantly increasing transcripts with potential roles in detoxification of host defenses included alcohol dehydrogenases and a group of unexpected transcripts whose products may play an, as yet, undiscovered role in host colonization by mountain pine beetle.
Date created
2013
DOI
doi:10.7939/R34F1MM6C
License information
Attribution 4.0 International
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Citation for previous publication
Robert JA, Pitt C, Bonnett TR, Yuen MMS, Keeling CI, Bohlmann J, Huber DPW. (2013). Disentangling detoxification: Gene expression analysis of feeding mountain pine beetle illuminates molecular-level host chemical defense detoxification mechanisms. PLOS One, 8(11), e77777. doi:10.1371/journal.pone.0077777.
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