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Permanent link (DOI): https://doi.org/10.7939/R3H98ZH5C

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A Simple Polymerase Chain Reaction-based Method for the Discrimination of Three Chicken Breeds Open Access

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Author or creator
Kubo, Y.
Plastow, G.
Mitsuhashi, T.
Additional contributors
Subject/Keyword
polymerase chain reaction
chicken
single nucleotide polymorphism
amplified fragment length polymorphism
breed discrimination
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
A large number of branded chicken products exist in Japan, and in some cases, the breed of chicken is an important factor used to attract consumer interest in the retail product. In order to establish a simple method for verifying such breed claims we applied the amplified fragment length polymorphism (AFLP) technique to nine chicken breeds (White Cornish, Red Cornish, White Plymouth Rock, New Hampshire, Rhode Island Red, Barred Plymouth Rock, Hinaidori, Tosajidori, Tsushimajidori) to search for molecular markers able to discriminate chicken breeds. Three breed-specific single nucleotide polymorphisms (SNP) were identified, one for each of Hinaidori, Tosajidori, or New Hampshire. A total of 219 individuals from the nine breeds were analyzed using a specific PCR test for each of these SNP. The PCR tests made it possible to discriminate between the breeds of chickens to identify products from these three breeds. This PCR method provides an efficient method for the routine analysis and verification of certified chicken products.
Date created
2009
DOI
doi:10.7939/R3H98ZH5C
License information
Rights
@2009 Kubo, Y., Plastow, G., Mitsuhashi, T. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
Citation for previous publication
Kubo, Y, Plastow, G., Mitsuhashi, T. (2009). A Simple Polymerase Chain Reaction-based Method for the Discrimination of Three Chicken Breeds. Asian-Australasian Journal of Animal Science, 22(9) 1241-1247.  http://dx.doi.org/10.5713/ajas.2009.80576

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