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Permanent link (DOI): https://doi.org/10.7939/R30P0X346

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Forward and Side Two Dimensional Scattered Light Patterns Studies of Single Cell for Label-Free Flow Cytometry Open Access

Descriptions

Other title
Subject/Keyword
Label-free
Light scattering study
VSEL
flow cytometry
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Shahin, Hesam
Supervisor and department
Wojciech Rozmus (Physics)
Ying Yin Tsui (Electrical and Computer Engineering)
Examining committee member and department
Robert Fedosejevs (Electrical and Computer Engineering)
Department
Department of Electrical and Computer Engineering
Specialization
Microsystems and Nanodevices
Date accepted
2016-01-19T13:53:04Z
Graduation date
2016-06
Degree
Master of Science
Degree level
Master's
Abstract
A technique using two dimensional scattered light patterns from single cells to extract useful information from cells has been investigated experimentally and numerically. The technique can be implemented in label-free cytometry as an alternative to conventional fluorescent-activated flow cytometry. In this thesis two dimensional scattered light patterns in both forward and side directions from latex beads with spherical and elliptical shapes, Hematopoietic Stem Cells and Very Small Embryonic Like Stem Cells have been studied. The ability to measure the patterns of a cell in forward and side direction simultaneously is an important step forward for such technique. In particular it has been demonstrated that useful information of a cell under investigation including the size and shape of its nucleus and its mitochondria distribution can be obtained from its simultaneously captured forward and side two dimensional scattered light patterns.
Language
English
DOI
doi:10.7939/R30P0X346
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
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