ERA

Download the full-sized PDF of The compact genome of the plant pathogen Plasmodiophora brassicae is adapted to intracellular interactions with host Brassica sppDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3HD7P608

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Agricultural, Food and Nutritional Science, Department of

Collections

This file is in the following collections:

Journal Articles (Agricultural, Food and Nutritional Science)

The compact genome of the plant pathogen Plasmodiophora brassicae is adapted to intracellular interactions with host Brassica spp Open Access

Descriptions

Author or creator
Rolfe, Stephen A.
Strelkov, Stephen E.
Links, Matthew G.
Clarke, Wayne E.
Robinson, Stephen J.
Djavaheri, Mohammad
Malinowski, Robert
Haddadi, Parham
Kagale, Sateesh
Parkin, Isobel A. P.
Taheri, Ali
Borhan, M. Hossein
Additional contributors
Subject/Keyword
Plasmodiophora brassicae
Clubroot
Brassica napus
Biotroph
Effectors
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Background The protist Plasmodiophora brassicae is a soil-borne pathogen of cruciferous species and the causal agent of clubroot disease of Brassicas including agriculturally important crops such as canola/rapeseed (Brassica napus). P. brassicae has remained an enigmatic plant pathogen and is a rare example of an obligate biotroph that resides entirely inside the host plant cell. The pathogen is the cause of severe yield losses and can render infested fields unsuitable for Brassica crop growth due to the persistence of resting spores in the soil for up to 20 years. Results To provide insight into the biology of the pathogen and its interaction with its primary host B. napus, we produced a draft genome of P. brassicae pathotypes 3 and 6 (Pb3 and Pb6) that differ in their host range. Pb3 is highly virulent on B. napus (but also infects other Brassica species) while Pb6 infects only vegetable Brassica crops. Both the Pb3 and Pb6 genomes are highly compact, each with a total size of 24.2 Mb, and contain less than 2 % repetitive DNA. Clustering of genome-wide single nucleotide polymorphisms (SNP) of Pb3, Pb6 and three additional re-sequenced pathotypes (Pb2, Pb5 and Pb8) shows a high degree of correlation of cluster grouping with host range. The Pb3 genome features significant reduction of intergenic space with multiple examples of overlapping untranslated regions (UTRs). Dependency on the host for essential nutrients is evident from the loss of genes for the biosynthesis of thiamine and some amino acids and the presence of a wide range of transport proteins, including some unique to P. brassicae. The annotated genes of Pb3 include those with a potential role in the regulation of the plant growth hormones cytokinin and auxin. The expression profile of Pb3 genes, including putative effectors, during infection and their potential role in manipulation of host defence is discussed. Conclusion The P. brassicae genome sequence reveals a compact genome, a dependency of the pathogen on its host for some essential nutrients and a potential role in the regulation of host plant cytokinin and auxin. Genome annotation supported by RNA sequencing reveals significant reduction in intergenic space which, in addition to low repeat content, has likely contributed to the P. brassicae compact genome.
Date created
2016
DOI
doi:10.7939/R3HD7P608
License information

Rights
Attribution 4.0 International
Citation for previous publication
Rolfe, S. A., Strelkov, S. E., Links, M. G., Clarke, W. E., Robinson, S. J., Djavaheri, M., Malinowski, R., Haddadi, P., Kagale, S., Parkin, I. A. P., Taheri, A., & Borhan, M. H. (2016). The compact genome of the plant pathogen Plasmodiophora brassicae is adapted to intracellular interactions with host Brassica spp. BMC Genomics, 17(272), [15 pages].  http://dx.doi.org/10.1186/s12864-016-2597-2

Source

Link to related item

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1831626
Last modified: 2017:09:06 16:10:01-06:00
Filename: BMCGeno_17_272.pdf
Original checksum: cb2a59663b546cec576618d7e87b8803
Well formed: true
Valid: false
Status message: Invalid destination object offset=1545886
Status message: Invalid destination object offset=1545886
Status message: Invalid destination object offset=1545886
Status message: Invalid destination object offset=1545886
Status message: Invalid destination object offset=1545886
File title: Abstract
File title: The compact genome of the plant pathogen Plasmodiophora brassicae is adapted to intracellular interactions with host Brassica spp
File author: Stephen A. Rolfe
Page count: 15
Activity of users you follow
User Activity Date