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Permanent link (DOI): https://doi.org/10.7939/R38P5VM30

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Effect of environmental exposure on infant gut microbiota composition and diversity in the Canadian Healthy Infant Longitudinal Development (CHILD) national birth cohort Open Access

Descriptions

Other title
Subject/Keyword
infant gut microbiota
environmental exposure
hospital acquired infections
household cleaning products
nosocomial pathogens
asthma allergic diseases
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Tun,Mon H
Supervisor and department
Kozyrskyj,Anita (Pediatrics Department)
Dinu, Irina (School of Public Health)
Examining committee member and department
Hicks, Elizabeth (Pediatrics Department(
Department
School of Public Health
Specialization
Epidemiology
Date accepted
2016-07-28T11:52:49Z
Graduation date
2016-06:Fall 2016
Degree
Master of Science
Degree level
Master's
Abstract
Background: The development of infant gut microbiota plays a crucial role in immune system development, and infant gut microbiota dysbiosis has been linked to asthma and allergic disease. Studies have shown that Bacteroidetes phylum was less abundant in the guts of allergic children. Although extensive research has been conducted to investigate the effects of delivery mode and infant feeding on gut mircobiota, not much is known about the influence of hospital microbial environment and exposure to household cleaning products on infant gut microbiota composition and diversity. Objective: To assess the impact of exposure to hospital microbial environment and household cleaning products, on the infant gut microbiota composition and diversity. Methods: Fecal samples from a subset of 787 children from the Edmonton, Winnipeg and Vancouver sites of the Canadian Healthy Infant Longitudinal Development (CHILD) birth cohort were included in the study. Infant hospital length-of-stay after birth was obtained from birth chart reviews and parent report at 3 months postpartum. Personal use of household cleaning products namely: disinfectant (multi-surface cleaner), detergent and other chemicals (spray air-freshener) were obtained from mothers at 3 months post-delivery. Infant gut microbiota at 3-4 months was characterized by Illumina 16S rRNA sequencing. Microbial relative abundance, Shannon diversity and Chao1 species richness were determined. Results: Newborn exposure to the hospital environment for more than one day was associated with higher fecal abundance, at infant age 3-4 months, of microbes belonging to the Lachnospiraceae family (p=0.041). In addition, Pseudomonadaceae (p=0.04) and genus Pseudomonas (p=0.027) were more abundant in the gut of infants with extended hospital stays after birth, in both vaginally and caesarean-delivered infants. High indoor disinfectant exposure was associated with low fecal abundance of Actinobacteria (p=0.0002) at the phylum level and of Bifidobacteriaceae (p=0.0003) at the family level. Moreover, total microbial diversity was reduced at the order level (p= 0.028) in infants in the high exposure group. Conclusion: The findings provide evidence of gut microbiota dysbiosis associated with exposure to hospital microbial environment and household cleaning products. The impact of these compositional changes on the development of gut immunity and atopic disease later in life requires further study.
Language
English
DOI
doi:10.7939/R38P5VM30
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
Citation for previous publication
Impact of early life hospitalization on infant gut microbiota composition at 3-4 months. Impact of postnatal exposure to household cleaning products on infant gut microbiota composition at 3-4 months

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