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Permanent link (DOI): https://doi.org/10.7939/R3NC5SF94

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A specialized ABC efflux transporter GcABC-G1 confers monoterpene resistance to Grosmannia clavigera, a bark beetle-associated fungal pathogen of pine trees Open Access

Descriptions

Author or creator
Wang, Ye
Lim, Lynette
DiGuistini, Scott
Robertson, Gordon
Bohlmann, Jörg
Breuil, Colette
Additional contributors
Subject/Keyword
mountain pine beetle (Dendroctonus ponderosae)
monoterpene
Grosmannia clavigera
lodgepole pine (Pinus contorta)
ABC transporter
Type of item
Journal Article (Published)
Language
english
Place
Time
Description
Grosmannia clavigera is a bark beetle-vectored pine pathogen in the mountain pine beetle epidemic in western North America. Grosmannia clavigera colonizes pines despite the trees' massive oleoresin terpenoid defences. We are using a functional genomics approach to identify G. clavigera's mechanisms of adaptation to pine defences. We annotated the ABC transporters in the G. clavigera genome and generated RNA-seq transcriptomes from G. clavigera grown with a range of terpenes. We functionally characterized GcABC-G1, a pleiotropic drug resistance (PDR) transporter that was highly induced by terpenes, using qRT-PCR, gene knock-out and heterologous expression in yeast. Deleting GcABC-G1 increased G. clavigera's sensitivity to monoterpenes and delayed development of symptoms in inoculated young lodgepole pine trees. Heterologous expression of GcABC-G1 in yeast increased tolerance to monoterpenes. G. clavigera but not the deletion mutant, can use (+)-limonene as a carbon source. Phylogenetic analysis placed GcABC-G1 outside the ascomycete PDR transporter clades. G. clavigera appears to have evolved two mechanisms to survive and grow when exposed to monoterpenes: GcABC-G1 controls monoterpene levels within the fungal cells and G. clavigera uses monoterpenes as a carbon source. This work has implications for understanding adaptation to host defences in an important forest insect–fungal system, and potentially for metabolic engineering of terpenoid production in yeast.
Date created
2013
DOI
doi:10.7939/R3NC5SF94
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© 2013 Wang et al. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
Citation for previous publication
Wang, Y., Lim, L., DiGuistini, S., Robertson, G., Bohlmann, J., Breuil, C. (2013). A specialized ABC efflux transporter GcABC-G1 confers monoterpene resistance to Grosmannia clavigera, a bark beetle-associated fungal pathogen of pine trees. New Phytologist, 197(3): 886-89. doi: 10.1111/nph.12063.
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File title: A specialized ABC efflux transporter GcABCG1 confers monoterpene resistance to Grosmannia clavigera, a bark beetleassociated fungal pathogen of pine trees
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