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Permanent link (DOI): https://doi.org/10.7939/R3PC2TP3Z

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Vaccenic acid suppresses intestinal inflammation by increasing the endocannabinoid anandamide and non-cannabinoid signaling molecules in a rat model of the metabolic syndrome Open Access

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Author or creator
Jacome-Sosa, Miriam
Vacca, Claudia
Mangat, Rabban
Diane, Abdoulaye
Nelson, Randy C.
Reaney, Martin J.
Shen, Jianheng
Curtis, Jonathan M.
Vine, Donna F.
Field, Catherine J.
Igarash, Miki
Piomelli, Daniele
Banni, Sebastiano
Proctor, Spencer D.
Additional contributors
Subject/Keyword
Endocannabinoids
Anandamide
N-acylethanolamines
Intestinal Inflammatory Diseases
Vaccenic Acid
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Vaccenic acid (VA), the predominant ruminant-derived trans fat in the food chain, ameliorates hyperlipidemia, yet mechanisms remain elusive. We investigated whether VA could influence tissue endocannabinoids (ECs) by altering the availability of their biosynthetic precursor, arachidonic acid (AA), in membrane phospholipids (PLs). JCR:LA-cp rats were assigned to a control diet with or without VA (1% w/w), cis-9, trans-11 conjugated linoleic acid (CLA) (1% w/w) or VA+CLA (1% + 0.5% w/w) for 8 weeks. VA reduced the EC, 2-arachidonoylglycerol (2-AG), in the liver and visceral adipose tissue (VAT) relative to control diet (P < 0.001), but did not change AA in tissue PLs. There was no additive effect of combining VA+CLA on 2-AG relative to VA alone (P > 0.05). Interestingly, VA increased jejunal concentrations of anandamide and those of the noncannabinoid signaling molecules, oleoylethanolamide and palmitoylethanolamide, relative to control diet (P < 0.05). This was consistent with a lower jejunal protein abundance (but not activity) of their degrading enzyme, fatty acid amide hydrolase, as well as the mRNA expression of TNFα and interleukin 1β (P < 0.05). The ability of VA to reduce 2-AG in the liver and VAT provides a potential mechanistic explanation to alleviate ectopic lipid accumulation. The opposing regulation of ECs and other noncannabinoid lipid signaling molecules by VA suggests an activation of benefit via the EC system in the intestine.
Date created
2016
DOI
doi:10.7939/R3PC2TP3Z
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Attribution 3.0 Unported
Citation for previous publication
Jacome-Sosa, M., Vacca, C., Mangat, R., Diane, A., Nelson, R. C., Reaney, M. J., Shen, J., Curtis, J. M., Vine, D. F., Field, C. J., Igarash, M., Piomelli, D., Banni, S., & Proctor, S. D. (2016). Vaccenic acid suppresses intestinal inflammation by increasing the endocannabinoid anandamide and non-cannabinoid signaling molecules in a rat model of the metabolic syndrome. Journal of Lipid Research, 57(4), 638-649.  http://dx.doi.org/10.1194/jlr.M066308

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File title: Vaccenic acid suppresses intestinal inflammation by increasing anandamide and related N-acylethanolamines in the JCR:LA-cp rat[S]
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