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Permanent link (DOI): https://doi.org/10.7939/R3WP9TM1Q

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Transcriptome microRNA profiling of bovine mammary epithelial cells challenged with Escherichia coli or Staphylococcus aureus bacteria reveals pathogen directed microRNA expression profiles Open Access

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Author or creator
Jin, Weiwu
Ibeagha-Awemu, Eveline M.
Liang, Guanxiang
Beaudoin, Frédéric
Zhao, Xin
Guan, Leluo
Additional contributors
Subject/Keyword
MicroRNA-Sequencing
S. aureus
Mastitis
Bovine Mammary Epithelial Cells
E. coli
MicroRNA
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Background MicroRNAs (miRNAs) can post-transcriptionally regulate gene expression and have been shown to be critical regulators to the fine-tuning of epithelial immune responses. However, the role of miRNAs in bovine responses to E. coli and S. aureus, two mastitis causing pathogens, is not well understood. Results The global expression of miRNAs in bovine mammary epithelial cells (MAC-T cells) challenged with and without heat-inactivated Staphylococcus aureus (S. aureus) or Escherichia coli (E. coli) bacteria at 0, 6, 12, 24, and 48 hr was profiled using RNA-Seq. A total of 231 known bovine miRNAs were identified with more than 10 counts per million in at least one of 13 libraries and 5 miRNAs including bta-miR-21-5p, miR-27b, miR-22-3p, miR-184 and let-7f represented more than 50% of the abundance. One hundred and thirteen novel miRNAs were also identified and more than one third of them belong to the bta-miR-2284 family. Seventeen miRNAs were significantly (P 
Date created
2014
DOI
doi:10.7939/R3WP9TM1Q
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Attribution 4.0 International
Citation for previous publication
Jin, W., Ibeagha-Awemu, E. M., Liang, G., Beaudoin, F., Zhao, X., & Guan, L. L. (2014). Transcriptome microRNA profiling of bovine mammary epithelial cells challenged with Escherichia coli or Staphylococcus aureus bacteria reveals pathogen directed microRNA expression profiles. BMC Genomics, 15(181), [16 pages].  http://dx.doi.org/10.1186/1471-2164-15-181

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Copyright note: � 2014 Jin et al.; licensee BioMed Central Ltd.
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