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Permanent link (DOI): https://doi.org/10.7939/R3VH5CX67

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Gene-expression profiling of calves 6 and 9 months after inoculation with Mycobacterium avium subspecies paratuberculosis Open Access

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Author or creator
David, Joel
Barkema, Herman W.
Guan, Leluo
De Buck, Jeroen
Additional contributors
Subject/Keyword
Antigen Presentation
SSP Paratuberculosis
Growth-Factor-Beta
Blood Mononuclear-Cells
Johnes-Disease
Lipid Mediators
Real-Time Pcr
Crohns-Disease
T-Lymphocyte Migration
Dendritic Cells
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Early detection of Johne's disease (JD) caused by Mycobacterium avium subspecies paratuberculosis (MAP) is essential to reduce transmission; consequently, new diagnostic techniques and approaches to detect MAP or markers of early MAP infection are being explored. The objective was to identify biomarkers associated with MAP infection at 6 and 9 months after oral inoculation. Therefore, gene expression analysis was done using whole blood cells obtained from MAP-infected calves. All MAP-inoculated calves had a cell-mediated immune response (IFN-γ) to Johnin PPD specific antigens, and 60% had an antibody response to MAP antigens. Gene expression analysis at 6 months after inoculation revealed downregulation of chemoattractants, namely neutrophil beta-defensin-9 like peptide (BNBD9-Like), S100 calcium binding protein A9 (s100A9) and G protein coupled receptor 77 (GPR77) or C5a anaphylatoxin chemotactic receptor (C5a2). Furthermore, BOLA/MHC-1 intracellular antigen presentation gene was downregulated 9 months after inoculation. In parallel, qPCR experiments to evaluate the robustness of some differentially expressed genes revealed consistent downregulation of BOLA/MHC-I, BNBD9-Like and upregulation of CD46 at 3, 6, 9, 12, and 15 months after inoculation. In conclusion, measuring the expression of these genes has potential for implementation in a diagnostic tool for the early detection of MAP infection.
Date created
2014
DOI
doi:10.7939/R3VH5CX67
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Attribution 4.0 International
Citation for previous publication
David, J., Barkema, H. W., Guan, L. L., & De Buck, J. (2014). Gene-expression profiling of calves 6 and 9 months after inoculation with Mycobacterium avium subspecies paratuberculosis. Veterinary Research, 45(96), [12 pages].  http://dx.doi.org/10.1186/s13567-014-0096-5

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Copyright note: � 2014 David et al.; licensee BioMed Central Ltd.
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