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Permanent link (DOI): https://doi.org/10.7939/R3QS22

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Proteomics indicators of the rapidly shifting physiology from whole mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae), adults during early host colonization Open Access

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Author or creator
Pitt, Caitlin
Robert, Jeanne A.
Bonnett, Tiffany R.
Keeling, Christopher I.
Bohlmann, Jörg
Huber, Dezene P. W.
Additional contributors
Subject/Keyword
Chaperone proteins
Beetles
Transcriptome analysis
Pines
Proteomes
Heat shock response
Carbohydrate metabolism
Protein metabolism
Type of item
Journal Article (Published)
Language
english
Place
Time
Description
We developed proteome profiles for host colonizing mountain pine beetle adults, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae). Adult insects were fed in pairs on fresh host lodgepole pine, Pinus contorta Dougl. ex Loud, phloem tissue. The proteomes of fed individuals were monitored using iTRAQ and compared to those of starved beetles, revealing 757 and 739 expressed proteins in females and males, respectively, for which quantitative information was obtained. Overall functional category distributions were similar for males and females, with the majority of proteins falling under carbohydrate metabolism (glycolysis, gluconeogenesis, citric acid cycle), structure (cuticle, muscle, cytoskeleton), and protein and amino acid metabolism. Females had 23 proteins with levels that changed significantly with feeding (p<0.05, FDR<0.20), including chaperones and enzymes required for vitellogenesis. In males, levels of 29 proteins changed significantly with feeding (p<0.05, FDR<0.20), including chaperones as well as motor proteins. Only two proteins, both chaperones, exhibited a significant change in both females and males with feeding. Proteins with differential accumulation patterns in females exhibited higher fold changes with feeding than did those in males. This difference may be due to major and rapid physiological changes occurring in females upon finding a host tree during the physiological shift from dispersal to reproduction. The significant accumulation of chaperone proteins, a cytochrome P450, and a glutathione S-transferase, indicate secondary metabolite-induced stress physiology related to chemical detoxification during early host colonization. The females' activation of vitellogenin only after encountering a host indicates deliberate partitioning of resources and a balancing of the needs of dispersal and reproduction.
Date created
2014
DOI
doi:10.7939/R3QS22
License information
Attribution 4.0 International
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Citation for previous publication
Pitt, C., Robert, J.A., Bonnett, T.R., Keeling, C.I., Bohlmann, J., Huber, D.P (2014). Proteomics indicators of the rapidly shifting physiology from whole mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae), adults during early host colonization. PLoS One, 9(10), e110673. DOI: 10.1371/journal.pone.0110673.
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