ERA

Download the full-sized PDF of Expansion of ruminant-specific microRNAs shapes target gene expression divergence between ruminant and non-ruminant speciesDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3P55DJ6Q

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Roy Berg Kinsella Research Ranch

Collections

This file is in the following collections:

Journal Articles (Kinsella Ranch)

Expansion of ruminant-specific microRNAs shapes target gene expression divergence between ruminant and non-ruminant species Open Access

Descriptions

Author or creator
Bao, H.
Kommadath, A.
Sun, X.
Meng, Y.
Arantes, A. S.
Plastow, G. S.
Guan, L. L.
Stothard, P.
Additional contributors
Subject/Keyword
diversity
drosophila
database
evolution
rna
animals
sequences
genome
alignment
metabolism
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Background Understanding how species-specific microRNAs (miRNAs) contribute to species-specific phenotypes is a central topic in biology. This study aimed to elucidate the role of ruminant-specific miRNAs in shaping mRNA expression divergence between ruminant and non-ruminant species. Results We analyzed miRNA and mRNA transcriptomes generated by Illumina sequencing from whole blood samples of cattle and a closely related non-ruminant species, pig. We found evidence of expansion of cattle-specific miRNAs by analyzing miRNA conservation among 57 vertebrate species. The emergence of cattle-specific miRNAs was accompanied by accelerated sequence evolution at their target sites. Further, the target genes of cattle-specific miRNAs show markedly reduced expression compared to their pig and human orthologues. We found that target genes with conserved or non-conserved target sites of cattle-specific miRNAs exhibit reduced expression. One of the significantly enriched KEGG pathway terms for the target genes of the cattle-specific miRNAs is the insulin signalling pathway, raising the possibility that some of these miRNAs may modulate insulin resistance in ruminants. Conclusions We provide evidence of rapid miRNA-mediated regulatory evolution in the ruminant lineage. Cattle-specific miRNAs play an important role in shaping gene expression divergence between ruminant and non-ruminant species, by influencing the expression of targets genes through both conserved and cattle-specific target sites.
Date created
2013
DOI
doi:10.7939/R3P55DJ6Q
License information
Attribution 4.0 International
Rights

Citation for previous publication
Bao, H., Kommadath, A., Sun, X., Meng, Y., Arantes, A.S., Plastow, G.S., Guan, L.L., Stothard, P. (2013). Expansion of ruminant-specific microRNAs shapes target gene expression divergence between ruminant and non-ruminant species. BMC Genomics 14(609).  http://dx.doi.org/10.1186/1471-2164-14-609

Source
Link to related item

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 532187
Last modified: 2016:06:24 18:08:20-06:00
Filename: BMC_GEN_14(609)
Original checksum: e14dfaf2ccbf7be7e1138e8a0de5a087
Copyright note: ??? 2013 Bao et al.; licensee BioMed Central Ltd.
Well formed: true
Valid: true
File title: Abstract
File title: Expansion of ruminant-specific microRNAs shapes target gene expression divergence between ruminant and non-ruminant species
Page count: 10
Activity of users you follow
User Activity Date