ERA

Download the full-sized PDF of Differential gene expression in femoral bone from red junglefowl and domestic chicken, differing for bone phenotypic traitsDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3C24QQ12

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Roy Berg Kinsella Research Ranch

Collections

This file is in the following collections:

Journal Articles (Kinsella Ranch)

Differential gene expression in femoral bone from red junglefowl and domestic chicken, differing for bone phenotypic traits Open Access

Descriptions

Author or creator
Rubin C-J, Lindberg J, Fitzsimmons, C.
Savolainen, P.
Jensen, P.
Lundeberg, J.
Andersson, L.
Kindmark, A.
Additional contributors
Subject/Keyword
bone and bones/metabolism
femur/metabolism
gene expression profiling
gene expression regulation
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Background Osteoporosis is frequently observed among aging hens from egg-producing strains (layers) of domestic chicken. White Leghorn (WL) has been intensively selected for egg production and it manifests striking phenotypic differences for a number of traits including several bone phenotypes in comparison with the wild ancestor of chicken, the red junglefowl (RJ). Previously, we have identified four Quantitative Trait Loci (QTL) affecting bone mineral density and bone strength in an intercross between RJ and WL. With the aim of further elucidating the genetic basis of bone traits in chicken, we have now utilized cDNA-microarray technology in order to compare global RNA-expression in femoral bone from adult RJ and WL (five of each sex and population). Results When contrasting microarray data for all WL-individuals to that of all RJ-individuals we observed differential expression (False discovery rate adjusted p-values < 0.015) for 604 microarray probes. In corresponding male and female contrasts, differential expression was observed for 410 and 270 probes, respectively. Altogether, the three contrasts between WL and RJ revealed differential expression of 779 unique transcripts, 57 of which are located to previously identified QTL-regions for bone traits. Some differentially expressed genes have previously been attributed roles in bone metabolism and these were: WNT inhibitory factor 1 (WIF1), WD repeat-containing protein 5 (WDR5) and Syndecan 3 (SDC3). Among differentially expressed transcripts, those encoding structural ribosomal proteins were highly enriched and all 15 had lower expression in WL. Conclusion We report the identification of 779 differentially expressed transcripts, several residing within QTL-regions for bone traits. Among differentially expressed transcripts, those encoding structural ribosomal proteins were highly enriched and all had lower expression levels in WL. In addition, transcripts encoding four translation initiation and translation elongation factor proteins also had lower expression levels in WL, possibly indicating perturbation of protein biosynthesis pathways between the two populations. Information derived from this study could be relevant to the bone research field and may also aid in further inference of genetic changes accompanying animal domestication.
Date created
2007
DOI
doi:10.7939/R3C24QQ12
License information
Attribution 4.0 International
Rights

Citation for previous publication
Rubin C-J, Lindberg J, Fitzsimmons, C., Savolainen P, Jensen P, Lundeberg J, Andersson L, & Kindmark A. (2007). Differential gene expression in femoral bone from red junglefowl and domestic chicken, differing for bone phenotypic traits. BMC Genomics 8(1), 208.  http://dx.doi.org/10.1186/1471-2164-8-208

Source
Link to related item

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1970812
Last modified: 2016:06:24 18:08:28-06:00
Filename: BMC_genomics_8(1)
Original checksum: 1c2c7958dcf9518e1c51fd303fa92d83
Well formed: true
Valid: false
Status message: Invalid destination object offset=1917183
Status message: Invalid destination object offset=1917183
Status message: Invalid destination object offset=1917183
Status message: Invalid destination object offset=1917183
Status message: Invalid destination object offset=1917183
File title: Abstract
File title: 1471-2164-8-208.fm
File author: petere
Page count: 23
Activity of users you follow
User Activity Date