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Permanent link (DOI): https://doi.org/10.7939/R3H70854D

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Analysis of the acute phase response in goldfish (Carassius auratus L.) infected with Trypanosoma carassii Open Access

Descriptions

Other title
Subject/Keyword
C-reactive protein
acute phase response
Trypanosoma carassii
acute phase proteins
goldfish
Serum Amyloid A
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Kovacevic, Nikolina
Supervisor and department
Belosevic, Miodrag (Biological Sciences)
Examining committee member and department
Hanington, Patrick (School of Public Health)
Keddie, Andrew (Biological Sciences)
Barreda, Daniel (Biological Sciences; Agriculture, Food & Nutritional Sciences)
Department
Department of Biological Sciences
Specialization
Physiology, Cell and Developmental Biology
Date accepted
2015-10-15T10:30:29Z
Graduation date
2016-06
Degree
Master of Science
Degree level
Master's
Abstract
The innate immune response is a fundamental defense mechanism in bony fishes and a crucial component of innate immunity is the acute phase response (APR). The APR is a systemic and/or local response to any injury, infection, or trauma and is characterized by a change in the blood composition of acute phase proteins (APPs). In both veterinary and human medicine, APPs are well characterized, however the functional significance of fish APP orthologs has not been fully elucidated. My master’s research focused on the immunological interactions between T. carassii and its goldfish host with a focus on the APR. The gene expression profile of several APPs in the kidney, liver and spleen of T. carassii infected goldfish was examined during the acute and chronic phases of infection. The genes encoding for C-reactive Protein (CRP) and Serum Amyloid A (SAA) were highly expressed in the tissues of goldfish during the course of T. carassii infection. Consequently, recombinant goldfish CRP and SAA were generated and functionally characterized. Recombinant goldfish CRP (rgfCRP) enhanced complement-mediated killing of trypanosomes in vitro, and the lysis of the parasites was enhanced after addition of immune serum to cultures. rgfCRP did not affect the production of reactive oxygen and nitrogen intermediates by monocytes and macrophages, respectively. Furthermore, unlike mammalian recombinant CRP, rgfCRP did not act as an opsonin to enhance phagocytosis of T. carassii by macrophages. Recombinant goldfish SAA (rgSAA) treated monocytes and macrophages exhibited differential gene expression of select immune genes. rgSAA induced gene expression of both pro-inflammatory (TNFα1, TNFα2) and anti-inflammatory cytokines (IL-10, TGFβ) in monocytes, and IL-1β1 and SAA gene expression macrophages. rgSAA was chemotactic to macrophages and neutrophils, but not monocytes. rgSAA had no effect on respiratory burst in monocytes, however, it suppressed nitric oxide production in macrophages exposed to heat-killed Aeromonas salmonicida. rgSAA displayed antibacterial properties against Escherichia coli in a concentration dependent manner. The results of my thesis research present the first comprehensive analysis of the acute phase response during the course of a protozoan infection of bony fish. Additionally, it also presents a first comprehensive analysis of two major acute phase proteins, CRP and SAA, in bony fish.
Language
English
DOI
doi:10.7939/R3H70854D
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
Citation for previous publication
N. Kovacevic, M.O. Hagen, J. Xie, M. Belosevic, The analysis of the acute phase response during the course of Trypanosoma carassii infection in the goldfish (Carassius auratus L.), Dev. Comp. Immunol. 53 (2015) 112–122

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