ERA

Download the full-sized PDF of Examining the role of the host helicase DDX56 during Flavivirus infectionsDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3639KH98

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Examining the role of the host helicase DDX56 during Flavivirus infections Open Access

Descriptions

Other title
Subject/Keyword
Flavivirus
Viral-host interactions
RNA Helicases
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Reid, Colleen R.
Supervisor and department
Hobman, Tom C. (Cell Biology)
Examining committee member and department
Evans, David (Medical Microbiology and Immunology)
Marchant, Davd (Medical Microbiology and Immunology)
Montpetit, Benjamen (Cell Biology)
Department
Department of Medical Microbiology and Immunology
Specialization
Virology
Date accepted
2016-01-06T15:27:38Z
Graduation date
2016-06
Degree
Master of Science
Degree level
Master's
Abstract
Flaviviruses, such as West Nile virus (WNV), Powassan virus (POW), and Saint Louis encephalitis virus (SLEV), are significant pathogens capable of causing serious neurological disease in both humans and animals. Despite their relevance to human health, treatment and vaccines for these viruses remain limited. Characterizing important cellular host factors during viral infection may identify novel targets for the development of antivirals. Previously, a host nucleolar RNA helicase, DDX56, was identified as an essential host factor for WNV infectivity by utilizing its ATP-dependent helical activity to enhance the packaging of viral RNA. Through extensive microscopy analyses I show that DDX56 localizes to the ER, the site of viral replication and assembly, during WNV infection and colocalizes with markers for viral assembly. Additionally, DDX56 does not play a role in the reorganization of membranes and the induction of viral structural elements in WNV infection. Together, these data support the role of DDX56 in assembly of infectious virions. I also investigated whether other viruses, POW and SLEV, utilize DDX56 during infection making it a potential target for broad-spectrum antivirals. Interestingly, while I discovered that these viruses affect DDX56 localization and stability, they do not require this helicase for the production of infectious virions. Thus, DDX56 seems to be an essential host factor for WNV while the underlying mechanism of relocalization may not be virus specific. Collectively, my studies further our understanding of the role of DDX56 during WNV infection and further support it as a target for the development of antivirals.
Language
English
DOI
doi:10.7939/R3639KH98
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2016-01-06T22:27:47.320+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 102254208
Last modified: 2016:10:29 00:18:52-06:00
Filename: Reid_Colleen_R_201512_MSc.pdf
Original checksum: ac6f8ea0251ba57793a5369d0c306271
Well formed: false
Valid: false
Status message: Invalid page tree node offset=101651281
Status message: Unexpected error in findFonts java.lang.ClassCastException: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject cannot be cast to edu.harvard.hul.ois.jhove.module.pdf.PdfDictionary offset=102226423
Status message: Malformed outline dictionary offset=102231398
Activity of users you follow
User Activity Date