ERA

Download the full-sized PDF of Inulin and fructo-oligosaccharides have divergent effects on colitis and commensal microbiota in HLA-B27 transgenic ratsDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3WS8J06D

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Agricultural, Food and Nutritional Science, Department of

Collections

This file is in the following collections:

Journal Articles (Agricultural, Food and Nutritional Science)

Inulin and fructo-oligosaccharides have divergent effects on colitis and commensal microbiota in HLA-B27 transgenic rats Open Access

Descriptions

Author or creator
Koleva, Petya
Valcheva, Rosica
Sun, Xu
Gänzle, Michael
Dieleman, Levinus
Additional contributors
Subject/Keyword
Quantitative PCR
Inulin
Colitis
Denaturing Gradient Gel Electrophoresis
Clostridium difficile
Fructo-Oligosaccharides
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Modulation of intestinal microbiota by non-digestible carbohydrates may reduce inflammation in inflammatory bowel disease (IBD). The aim of the present study was to assess the effects of inulin and fructo-oligosaccharides (FOS) on intestinal microbiota and colitis in HLA-B27 transgenic rats, a well-validated rodent model for IBD. In this study, 4-week-old rats were fed 8 g/kg body weight inulin or FOS for 12 weeks, or not. Faeces were collected at 4 and 16 weeks of age; and caecal samples were collected at necropsy. The effects of inulin and FOS on chronic intestinal inflammation were assessed using a gross gut score, histology score and levels of mucosal IL-1β. Intestinal microbiota were characterised by quantitative PCR and denaturing gradient gel electrophoresis. Colitis was significantly reduced in all FOS-fed rats compared to the control diet, whereas inulin decreased chronic intestinal inflammation in only half the number of animals. Quantitative analysis of caecal microbiota demonstrated that inulin increased the numbers of total bacteria and the Bacteroides-Prevotella-Porphyromonas group, FOS increased bifidobacteria, and both fructans decreased Clostridium cluster XI. In the faecal samples, both inulin and FOS decreased total bacteria, Bacteroides-Prevotella-Porphyromonas group, and Clostridium clusters XI and XIVa. FOS increased Bifidobacterium spp., and mediated a decrease of gene copies of Enterobacteriaceae and Clostridium difficile toxin B in faeces. SCFA concentrations in the faecal and caecal samples were unaffected by the diets. In conclusion, FOS increased the abundance of Bifidobacterium spp., whereas both fructans reduced Clostridium cluster XI and C. difficile toxin gene expression, correlating with a reduction of chronic intestinal inflammation.
Date created
2012
DOI
doi:10.7939/R3WS8J06D
License information
© The Authors 2012
Rights

Citation for previous publication
Koleva, P., Valcheva, R., Sun, X., Gänzle, M., & Dieleman, L. (2012). Inulin and fructo-oligosaccharides have divergent effects on colitis and commensal microbiota in HLA-B27 transgenic rats. British Journal of Nutrition, 108(9), 1633-1643.  http://dx.doi.org/10.1017/S0007114511007203

Source

Link to related item

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 199634
Last modified: 2017:09:06 16:04:46-06:00
Filename: BJN_108_9_1633.pdf
Original checksum: d91032058028c7508932c56f533ea62d
Well formed: true
Valid: true
File title: 406446 1633..1643
Page count: 11
Activity of users you follow
User Activity Date