Download the full-sized PDF
Permanent link (DOI): https://doi.org/10.7939/R34J0B94Q
This file is in the following communities:
|Chemistry, Department of|
This file is in the following collections:
|Journal Articles (Chemistry)|
Tulane virus recognizes sialic acids as cellular receptors Open Access
- Author or creator
Klassen, John S.
- Additional contributors
- Type of item
- Journal Article (Published)
The recent discovery that human noroviruses (huNoVs) recognize sialic acids (SAs) in addition to histo-blood group antigens (HBGAs) pointed to a new direction in studying virus-host interactions during calicivirus infection. HuNoVs remain difficult to study due to the lack of an effective cell culture model. In this study, we demonstrated that Tulane virus (TV), a cultivable primate calicivirus, also recognizes SAs in addition to the previously known TV-HBGA interactions. Evidence supporting this discovery includes that TV virions bound synthetic sialoglycoconjugates (SGCs) and that treatment of TV permissive LLC-MK2 cells with either neuraminidases or SA-binding lectins inhibited TV infectivity. In addition, we found that Maackia amurensis leukoagglutinin (MAL), a lectin that recognizes the α-2,3 linked SAs, bound LLC-MK2 cells, as well as TV, by which MAL promoted TV infectivity in cell culture. Our findings further highlight TV as a valuable surrogate for huNoVs, particularly in studying virus-host interactions that may involve two host carbohydrate receptors or co-receptors for infection.
- Date created
- License information
- Attribution 4.0 International
- Citation for previous publication
Tan, M., Xia, M., Wei, C., Fang, H., Quigley, C., Huang, P., Zhong, W., Zhang, X., Fan, Q., Klassen, J., and Jiang, X. (2015). Tulane virus recognizes sialic acids as cellular receptors. Scientific Reports, 5(July), 11784 [14 pages].
- Link to related item
- Date Uploaded
- Date Modified
- Audit Status
- Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1880823
Last modified: 2017:06:24 01:01:45-06:00
Original checksum: 4495ec5f5084fad252af2b4b3878c11b
Copyright note: © 2015 Nature Publishing Group
File title: Tulane virus recognizes sialic acids as cellular receptors
File author: Ming Tan
Page count: 14