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Permanent link (DOI): https://doi.org/10.7939/R3JD67

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Electrografted Thick Diazonium Derived Films for Biosensing Applications Open Access

Descriptions

Other title
Subject/Keyword
Biosensing
Diazonium
SPR
Electrografting
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Toman, John T
Supervisor and department
McDermott, Mark (Chemistry)
Examining committee member and department
Campbell, Robert (Chemistry)
Serpe, Michael (Chemistry)
Department
Department of Chemistry
Specialization

Date accepted
2013-01-29T10:33:39Z
Graduation date
2013-06
Degree
Master of Science
Degree level
Master's
Abstract
Electrografting permits bonding of an organic film to a conductive substrate. It is therefore important to control the formation of organic films as best as possible and to understand the linkage between the organic film and the substrate as best as possible. This work explores electrografting of diazonium salts using high reduction potentials to prepare thick aryl films as substrates for SPR immunoassays. Film thickness was linear with respect to applied reduction potential for the modification of gold electrodes with phenylacetic acid and nitroazobenzene diazonium salts. Further, the presence of redox active functional groups was determined to be unnecessary due to the large driving force of the reaction. Phenylacetic acid films were shown to provide a suitable platform for antibody immobilization and antigen binding, with LOD’s comparable to other SPR based biosensors. The immobilization of antibodies and subsequent antigen binding was shown to be highly dependent on surface morphology.
Language
English
DOI
doi:10.7939/R3JD67
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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