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Permanent link (DOI): https://doi.org/10.7939/R3804XZ7Z

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Identifying carbohydrate ligands of a norovirus P particle using a catch and release electrospray ionization mass spectrometry assay Open Access

Descriptions

Author or creator
Han, Ling
Kitova, Elena N.
Tan, Ming
Jiang, Xi
Klassen, John S.
Additional contributors
Subject/Keyword
Electrospray ionization mass spectrometry
Carbohydrate
P particle
Norovirus
Catch and release
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Noroviruses (NoVs), the major cause of epidemic acute gastroenteritis, recognize human histo-blood group antigens (HBGAs), which are present as free oligosaccharides in bodily fluid or glycolipids and glycoproteins on the surfaces of cells. The subviral P particle formed by the protruding (P) domain of the NoV capsid protein serves as a useful model for the study NoV–HBGA interactions. Here, we demonstrate the application of a catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS) assay for screening carbohydrate libraries against the P particle to rapidly identify NoV ligands and potential inhibitors. Carbohydrate libraries of 50 and 146 compounds, which included 18 and 24 analogs of HBGA receptors, respectively, were screened against the P particle of VA387, a member of the predominant GII.4 NoVs. Deprotonated ions corresponding to the P particle bound to carbohydrates were isolated and subjected to collision-induced dissociation to release the ligands in their deprotonated forms. The released ligands were identified by ion mobility separation followed by mass analysis. All 13 and 16 HBGA ligands with intrinsic affinities >500 M–1 were identified in the 50 and the 146 compound libraries, respectively. Furthermore, screening revealed interactions with a series of oligosaccharides with structures found in the cell wall of mycobacteria and human milk. The affinities of these newly discovered ligands are comparable to those of the HBGA receptors, as estimated from the relative abundance of released ligand ions.
Date created
2014
DOI
doi:10.7939/R3804XZ7Z
License information
© 2014 Han, L., Kitova, E. N., Tan, M., Jiang, X., & Klassen, J. S. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
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Citation for previous publication
Han, L., Kitova, E. N., Tan, M., Jiang, X., & Klassen, J. S. (2014). Identifying carbohydrate ligands of a norovirus P particle using a catch and release electrospray ionization mass spectrometry assay. Journal of the American Society for Mass Spectrometry, 25(1), 111-119.  http://doi.org/10.1007/s13361-013-0752-4

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