ERA

Download the full-sized PDF of Irinotecan (CPT-11) chemotherapy alters intestinal microbiota in tumour bearing ratsDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3ST7F97Q

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Agricultural, Food and Nutritional Science, Department of

Collections

This file is in the following collections:

Journal Articles (Agricultural, Food and Nutritional Science)

Irinotecan (CPT-11) chemotherapy alters intestinal microbiota in tumour bearing rats Open Access

Descriptions

Author or creator
Lin, Xiaoxi
Dieleman, Levinus A.
Ketabi, Ali
Bibova, Ilona
Sawyer, Michael B.
Xue, Hongyu
Field, Catherine J.
Baracos, Vickie E.
Gänzle, Michael G.
Additional contributors
Subject/Keyword
Gastrointestinal Tract
Clostridium
Clostridium difficile
Glutamine
Cancer Treatment
Diarrhea
Chemotherapy
Microbiome
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Intestinal microbiota mediate toxicity of irinotecan (CPT-11) cancer therapies and cause systemic infection after CPT-11-induced loss of barrier function. The intestinal microbiota and their functions are thus potential targets for treatment to mitigate CPT-11 toxicity. However, microbiota changes during CPT-11 therapy remain poorly described. This study analysed changes in intestinal microbiota induced by CPT-11 chemotherapy. Qualitative and quantitative taxonomic analyses, and functional analyses were combined to characterize intestinal microbiota during CPT-11-based chemotherapy, and in presence or absence of oral glutamine, a treatment known to reduce CPT-11 toxicity. In the first set of experiments tumour-bearing rats received a dose-intensive CPT-11 regimen (125 mg kg−1×3 days), with or without oral glutamine bolus (0.75 g kg−1). In a subsequent more clinically-oriented chemotherapy regimen, rats received two cycles of CPT-11 (50 mg kg−1) followed by 5-flurouracil (50 mg kg−1). The analysis of fecal samples over time demonstrated that tumours changed the composition of intestinal microbiota, increasing the abundance of clostrridial clusters I, XI, and Enterobacteriaceae. CPT-11 chemotherapy increased cecal Clostridium cluster XI and Enterobacteriaceae, particularly after the dose-intensive therapy. Glutamine treatment prevented the reduced abundance of major bacterial groups after CPT-11 administration; i.e. total bacteria, Clostridium cluster VI, and the Bacteroides-group. Virulence factor/toxin genes of pathogenic Escherichia coli and Clostridium difficile were not detected in the cecal microbiota. In conclusion, both colon cancer implantation and CPT-11-based chemotherapies disrupted the intestinal microbiota. Oral glutamine partially mitigated CPT-11 toxicity and induced temporary changes of the intestinal microbiota.
Date created
2012
DOI
doi:10.7939/R3ST7F97Q
License information

Rights
Attribution 4.0 International
Citation for previous publication
Lin, X., Dieleman, L. A., Ketabi, A., Bibova, I., Sawyer, M. B., Xue, H., Field, C. J., Baracos, V. E., & Gänzle, M. G. (2012). Irinotecan (CPT-11) chemotherapy alters intestinal microbiota in tumour bearing rats. PLoS ONE, 7(7), e39764 [8 pages].  http://dx.doi.org/10.1371/journal.pone.0039764

Source

Link to related item

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 470750
Last modified: 2017:09:06 16:39:36-06:00
Filename: PLoSONE_7_7_e39764.PDF
Original checksum: c1650e67808cabd0526a311544f9c2a1
Well formed: true
Valid: true
File title: deb_pone.0039764 1..8
Page count: 8
Activity of users you follow
User Activity Date