ERA

Download the full-sized PDF of The role of water channel proteins in facilitating recovery of leaf hydraulic concutance from water stress in Populus trichocarpaDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3D795Q4T

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Renewable Resources, Department of

Collections

This file is in the following collections:

Journal Articles (Renewable Resources)

The role of water channel proteins in facilitating recovery of leaf hydraulic concutance from water stress in Populus trichocarpa Open Access

Descriptions

Author or creator
Laur, Joan
Hacke, Uwe G.
Additional contributors
Subject/Keyword
Dehydration (Medicine)
Leaves
Membrane Proteins
Water Resources
Plant Biochemistry
Xylem
Gene Expression
Leaf Veins
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Gas exchange is constrained by the whole-plant hydraulic conductance (Kplant). Leaves account for an important fraction of Kplant and may therefore represent a major determinant of plant productivity. Leaf hydraulic conductance (Kleaf) decreases with increasing water stress, which is due to xylem embolism in leaf veins and/or the properties of the extra-xylary pathway. Water flow through living tissues is facilitated and regulated by water channel proteins called aquaporins (AQPs). Here we assessed changes in the hydraulic conductance of Populus trichocarpa leaves during a dehydration-rewatering episode. While leaves were highly sensitive to drought, Kleaf recovered only 2 hours after plants were rewatered. Recovery of Kleaf was absent when excised leaves were bench-dried and subsequently xylem-perfused with a solution containing AQP inhibitors. We examined the expression patterns of 12 highly expressed AQP genes during a dehydration-rehydration episode to identify isoforms that may be involved in leaf hydraulic adjustments. Among the AQPs tested, several genes encoding tonoplast intrinsic proteins (TIPs) showed large increases in expression in rehydrated leaves, suggesting that TIPs contribute to reversing drought-induced reductions in Kleaf. TIPs were localized in xylem parenchyma, consistent with a role in facilitating water exchange between xylem vessels and adjacent living cells. Dye uptake experiments suggested that reversible embolism formation in minor leaf veins contributed to the observed changes in Kleaf.
Date created
2014
DOI
doi:10.7939/R3D795Q4T
License information
Attribution 4.0 International
Rights

Citation for previous publication
Laur, J., and Hacke, U. (2014). The role of water channel proteins in facilitating recovery of leaf hydraulic concutance from water stress in Populus trichocarpa. PLoS ONE, 9(11), e111751 [10 pages].
Source

Link to related item

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 2841804
Last modified: 2017:09:05 16:34:47-06:00
Filename: PLoSONE_9_11_e111751.pdf
Original checksum: 40a6c317e0f40e4ea44b00832ac97fb2
Well formed: true
Valid: true
File title: gar_pone.0111751 1..10
Page count: 10
Activity of users you follow
User Activity Date