ERA

Download the full-sized PDF of A high resolution radiation hybrid map of bovine chromosome 14 identifies scaffold rearrangement in the latest bovine assemblyDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R36M3372D

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Roy Berg Kinsella Research Ranch

Collections

This file is in the following collections:

Journal Articles (Kinsella Ranch)

A high resolution radiation hybrid map of bovine chromosome 14 identifies scaffold rearrangement in the latest bovine assembly Open Access

Descriptions

Author or creator
Marques, E.
de Givry, S.
Stothard, P.
Murdoch, B.
Wang, Z.
Womack, J.
Moore, S. S.
Additional contributors
Subject/Keyword
pair 8/genetics
chromosomes
mammalian/genetics
mapping/methods
human
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Background Radiation hybrid (RH) maps are considered to be a tool of choice for fine mapping closely linked loci, considering that the resolution of linkage maps is determined by the number of informative meiosis and recombination events which may require very large mapping populations. Accurately defining the marker order on chromosomes is crucial for correct identification of quantitative trait loci (QTL), haplotype map construction and refinement of candidate gene searches. Results A 12 k Radiation hybrid map of bovine chromosome 14 was constructed using 843 single nucleotide polymorphism markers. The resulting map was aligned with the latest version of the bovine assembly (Btau_3.1) as well as other previously published RH maps. The resulting map identified distinct regions on Bovine chromosome 14 where discrepancies between this RH map and the bovine assembly occur. A major region of discrepancy was found near the centromere involving the arrangement and order of the scaffolds from the assembly. The map further confirms previously published conserved synteny blocks with human chromosome 8. As well, it identifies an extra breakpoint and conserved synteny block previously undetected due to lower marker density. This conserved synteny block is in a region where markers between the RH map presented here and the latest sequence assembly are in very good agreement. Conclusion The increase of publicly available markers shifts the rate limiting step from marker discovery to the correct identification of their order for further use by the research community. This high resolution map of bovine chromosome 14 will facilitate identification of regions in the sequence assembly where additional information is required to resolve marker ordering.
Date created
2007
DOI
doi:10.7939/R36M3372D
License information
Attribution 4.0 International
Rights

Citation for previous publication
Marques, E., S. de Givry,Stothard, P., B. Murdoch, Wang, Z., J. Womack, and S. S. Moore. (2007). A high resolution radiation hybrid map of bovine chromosome 14 identifies scaffold rearrangement in the latest bovine assembly. BMC Genomics, 8(254) 10 pg.  http://dx.doi.org/10.1186/1471-2164-8-254

Source
Link to related item

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 2091664
Last modified: 2016:06:24 17:30:54-06:00
Filename: bmc_genomics_8(310)
Original checksum: d2177b0750562d6a949ad8e3f44cf5ae
Well formed: true
Valid: false
Status message: Invalid destination object offset=2076553
Status message: Invalid destination object offset=2076553
Status message: Invalid destination object offset=2076553
Status message: Invalid destination object offset=2076553
Status message: Invalid destination object offset=2076553
File title: Abstract
File title: 1471-2164-8-310.fm
File author: productiontemp2
Page count: 10
Activity of users you follow
User Activity Date