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Permanent link (DOI): https://doi.org/10.7939/R33N20J1R

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Proteome differences associated with fat accumulation in bovine subcutaneous adipose tissues Open Access

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Author or creator
Zhao, Y. M.
Basu, U.
Dodson, M. V.
Basarab, J. A.
Guan, L.
Additional contributors
Subject/Keyword
adipogenesis
malic enzyme
preadipocyte differentiation
adipocyte
cattle
gene-expression profiles
skeletal-muscle
acid
perimuscular preadipocytes
proteins
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Background The fat components of red meat products have been of interest to researchers due to the health aspects of excess fat consumption by humans. We hypothesized that differences in protein expression have an impact on adipose tissue formation during beef cattle development and growth. Therefore, in this study we evaluated the differences in the discernable proteome of subcutaneous adipose tissues of 35 beef crossbred steers [Charolais × Red Angus (CHAR) (n = 13) and Hereford × Angus (HEAN) (n = 22)] with different back fat (BF) thicknesses. The goal was to identify specific protein markers that could be associated with adipose tissue formation in beef cows. Results Approximately 541-580 protein spots were detected and compared in each crossbred group, and 33 and 36 protein spots showed expression differences between tissues with high and low BF thicknesses from HEAN and CHAR crossbed, respectively. The annexin 1 protein was highly expressed in both crossbred steers that had a higher BF thickness (p < 0.05) and this was further validated by a western blot analysis. In 13 tissues of CHAR animals and 22 tissues of HEAN animals, the relative expression of annexin 1 was significantly different (p < 0.05) between tissues with high and low BF thicknesses. Conclusion The increased expression of annexin 1 protein has been found to be associated with higher BF thickness in both crossbred steers. This result lays the foundation for future studies to develop the protein marker for assessing animals with different BF thickness.
Date created
2010
DOI
doi:10.7939/R33N20J1R
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Attribution 4.0 International
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Zhao, Y.M., Basu, U., M.V. Dodson, Basarab, J.A. and L.Guan, L. (2010). Proteome differences associated with fat accumulation in bovine subcutaneous adipose tissues. Proteome Science 8(14), 14-25.  http://www.proteomesci.com/content/8/1/14

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File format: pdf (Portable Document Format)
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File title: Abstract
File title: Proteome differences associated with fat accumulation in bovine subcutaneous adipose tissues
File author: Yong Mei Zhao
Page count: 11
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