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Permanent link (DOI): https://doi.org/10.7939/R3N29PH8Q

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Method and evaluation thereof of yeast extract separation on the growth of microalgae Open Access

Descriptions

Other title
Subject/Keyword
microalgae
separation
yeast extract
solvent extraction
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Volk, Andrew James Alexander
Supervisor and department
McCaffrey, William (Chemical Engineering)
Burrell, Robert (Chemical Engineering and Biomedical Engineering)
Examining committee member and department
McCaffrey, William (Chemical Engineering)
Burrell, Robert (Chemical Engineering and Biomedical Engineering)
Sauvageau, Dominic (Chemical Engineering)
Department
Department of Chemical and Materials Engineering
Specialization
Chemical Engineering
Date accepted
2014-06-16T10:47:03Z
Graduation date
2014-11
Degree
Master of Science
Degree level
Master's
Abstract
Microalgae grow heterotrophically and produce high value pigments, nutraceuticals, pharmaceuticals, feeds, and biofuels. The growth rate, nutrient medium, growth density, and oil content strongly affect the economics of heterotrophic microalgae fermentation systems. This thesis explores the growth promoting properties of yeast extract, a complex media component, on the growth of Auxenochlorella protothecoides by solvent separation of yeast extract. Complete yeast extract was found to increase the growth rate of A. protothecoides, but the effect saturates above initial media concentrations of 4 g/L. Yeast extract partitions in several solvents. In methanol, the yeast extract partially dissolves. The solid portion boosts the initial growth rate of A. protothecoides above that of complete yeast extract by 149.4% in cell count and 24.7% in dry weight. The isopropanol/water separation is a liquid/liquid separation and produces a denser extract fraction (IT) that promotes the initial cell count of A. protothecoides by 55.6% above the maximal effect of yeast extract.
Language
English
DOI
doi:10.7939/R3N29PH8Q
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
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