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Influence of water deficit on the molecular responses of Pinus contorta × Pinus banksiana mature trees to infection by the mountain pine beetle fungal associate, Grosmannia clavigera Open Access

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Author or creator
Arango-Velez, Adriana
Galindo González, Leonardo M.
Meents, Miranda J.
Kayal, Walid El
Cooke, Barry J.
Linsky, Jean
Lusebrink, Inka
Cooke, Janice E.K.
Additional contributors
Subject/Keyword
naïve host
aquaporin
blue stain fungi
DREB
lodgepole pine
systemic
chitinase
jack pine
Dendroctonus ponderosae
range expansion
drought
terpene synthase
Type of item
Journal Article (Published)
Language
english
Place
Time
Description
Conifers exhibit a number of constitutive and induced mechanisms to defend against attack by pests and pathogens such as mountain pine beetle (Dendroctonus ponderosae Hopkins) and their fungal associates. Ecological studies have demonstrated that stressed trees are more susceptible to attack by mountain pine beetle than their healthy counterparts. In this study, we tested the hypothesis that water deficit affects constitutive and induced responses of mature lodgepole pine × jack pine hybrids (Pinus contorta Dougl. ex Loud. var. latifolia Engelm. ex S. Wats. × Pinus banksiana Lamb.) to inoculation with the mountain pine beetle fungal associate Grosmannia clavigera (Robinson-Jeffrey and Davidson) Zipfel, de Beer and Wingfield. The degree of stress induced by the imposed water-deficit treatment was sufficient to reduce photosynthesis. Grosmannia clavigera-induced lesions exhibited significantly reduced dimensions in water-deficit trees relative to well-watered trees at 5 weeks after inoculation. Treatment-associated cellular-level changes in secondary phloem were also observed. Quantitative RT-PCR was used to analyze transcript abundance profiles of 18 genes belonging to four families classically associated with biotic and abiotic stress responses: aquaporins (AQPs), dehydration-responsive element binding (DREB), terpene synthases (TPSs) and chitinases (CHIs). Transcript abundance profiles of a TIP2 AQP and a TINY-like DREB decreased significantly in fungus-inoculated trees, but not in response to water deficit. One TPS, Pcb(+)-3-carene synthase, and the Class II CHIs PcbCHI2.1 and PcbCHI2.2 showed increased expression under water-deficit conditions in the absence of fungal inoculation, while another TPS, Pcb(E)-β-farnesene synthase-like, and two CHIs, PcbCHI1.1 and PcbCHI4.1, showed attenuated expression under water-deficit conditions in the presence of fungal inoculation. The effects were observed both locally and systemically. These results demonstrate that both constitutive and induced carbon- and nitrogen-based defenses are affected by water deficit, suggesting potential consequences for mountain pine beetle dynamics, particularly in novel environments.
Date created
2013
DOI
doi:10.7939/R3JM23J63
License information
Rights
© 2013 Adriana Arango-Velez et al. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
Citation for previous publication
Arango-Velez, A., Meents, M.J., Galindo-González, L.M., El Kayal, W., Cooke, B.J., Linsky, J., Lusebrink, I., Cooke, J.E.K. (2013). Influence of water deficit on the molecular responses of Pinus contorta × Pinus banksiana mature trees to infection by the mountain pine beetle fungal associate, Grosmannia clavigera. Tree Physiology, 00, 1-20. doi:10.1093/treephys/tpt101.
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