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Permanent link (DOI): https://doi.org/10.7939/R3V40KC5C

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Arabidopsis GPAT9 contributes to synthesis of intracellular glycerolipids but not surface lipids Open Access

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Author or creator
Singer, Stacy D.
Chen, Guanqun
Mietkiewska, Elzbieta
Tomasi, Pernell
Jayawardhane, Kethmi
Dyer, John M.
Weselake, Randall J.
Additional contributors
Subject/Keyword
Acyl Lipid Biosynthesis
Acyl-CoA Specificity
Regio-Specificity
GPAT
Cutin
Lipid Droplet
Pollen Grain
Surface Wax
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (GPAT) genes encode enzymes involved in glycerolipid biosynthesis in plants. Ten GPAT homologues have been identified in Arabidopsis. GPATs 4–8 have been shown to be involved in the production of extracellular lipid barrier polyesters. Recently, GPAT9 was reported to be essential for triacylglycerol (TAG) biosynthesis in developing Arabidopsis seeds. The enzymatic properties and possible functions of GPAT9 in surface lipid, polar lipid and TAG biosynthesis in non-seed organs, however, have not been investigated. Here we show that Arabidopsis GPAT9 exhibits sn-1 acyltransferase activity with high specificity for acyl-coenzyme A, thus providing further evidence that this GPAT is involved in storage lipid biosynthesis. We also confirm a role for GPAT9 in seed oil biosynthesis and further demonstrate that GPAT9 contributes to the biosynthesis of both polar lipids and TAG in developing leaves, as well as lipid droplet production in developing pollen grains. Conversely, alteration of constitutive GPAT9 expression had no obvious effects on surface lipid biosynthesis. Taken together, these studies expand our understanding of GPAT9 function to include modulation of several different intracellular glycerolipid pools in plant cells.
Date created
2016
DOI
doi:10.7939/R3V40KC5C
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Attribution 4.0 International
Citation for previous publication
Singer, S. D., Chen, G., Mietkiewska, E., Tomasi, P., Jayawardhane, K., Dyer, J. M., & Weselake, R. J. (2016). Arabidopsis GPAT9 contributes to synthesis of intracellular glycerolipids but not surface lipids. Journal of Experimental Botany, 67(15), 4627-4638.  http://dx.doi.org/10.1093/jxb/erw242

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