ERA

Download the full-sized PDF of IMMOBILIZATION OF OLIGOSACCHARIDES IN AFFINITY CHROMATOGRAPHY AND APPLICATIONS TO SCREENING HUMAN MILK OLIGOSACCHARIDE RECEPTORSDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3R20S82F

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

IMMOBILIZATION OF OLIGOSACCHARIDES IN AFFINITY CHROMATOGRAPHY AND APPLICATIONS TO SCREENING HUMAN MILK OLIGOSACCHARIDE RECEPTORS Open Access

Descriptions

Other title
Subject/Keyword
p-toluenesulfonyl hydrazide
Human Milk Oligosaccharides
Affinity chromatography
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Hernandez Armada, Daniel
Supervisor and department
Cairo, Christopher W. (Chemistry)
Examining committee member and department
Klassen, John S. (Chemistry)
West, Frederick G. (Chemistry)
Department
Department of Chemistry
Specialization

Date accepted
2015-03-27T15:16:09Z
Graduation date
2015-06
Degree
Master of Science
Degree level
Master's
Abstract
A variety of applications in glycobiology exploit affinity chromatography through the immobilization of glycans to a solid support. Although several strategies are available, in many cases there is a lack of satisfactory chemical characterization. In this thesis we describe work towards improved characterization of chemistries with improved efficiencies which may be essential for work with glycans available only in limited quantity. Several chemical approaches were compared to divinyl sulfone based on immobilization efficiency, attachment to the solid support, and binding capacity to a protein receptor. We observed that immobilizations using p-Toluene sulfonyl hydrazide offered higher immobilization efficiencies and comparable binding capacities to the DVS method. We also generated a series of affinity matrices containing individual human milk oligosaccharides (HMO) species. To test the utility of the affinity matrix for identifying glycan receptors, we used the lysate from human cells. Proteins which specifically bound to the affinity matrix were captured, and then identified using LC-MS/MS-based proteomics analysis. This method has successfully identified a number of HMO-receptors, including members if the galectin family.
Language
English
DOI
doi:10.7939/R3R20S82F
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2015-03-27T21:16:10.505+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (PDF/A)
Mime type: application/pdf
File size: 3694821
Last modified: 2016:10:29 00:36:28-06:00
Filename: Hernandez Armada_Daniel_201503_MSc.pdf
Original checksum: 95d64bf7ef7206e74ff8cecc87d69060
Activity of users you follow
User Activity Date