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Permanent link (DOI): https://doi.org/10.7939/R3599Z447

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Gene discovery for the bark beetle-vectored fungal tree pathogen Grosmannia clavigera Open Access

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Author or creator
Hesse-Orce, Uljana
DiGuistini, Scott
Keeling, Christopher I.Wang, Ye
Li, Maria
Henderson, Hannah
Docking, Roderick T.
Liao, Nancy Y.
Robertson, Gordon
Holt, Robert A.
Jones, Steven J. M.
Bohlmann, Jörg
Breuil, Colette
Additional contributors
Subject/Keyword
terpenoid and phenolic defense compounds
fungal oxidative stress response
ophiostomatoid fungi
bark beetle-vectored fungal pathogen
Grosmannia clavigera
LPPE treatment
Type of item
Journal Article (Published)
Language
english
Place
Time
Description
Background Grosmannia clavigera is a bark beetle-vectored fungal pathogen of pines that causes wood discoloration and may kill trees by disrupting nutrient and water transport. Trees respond to attacks from beetles and associated fungi by releasing terpenoid and phenolic defense compounds. It is unclear which genes are important for G. clavigera's ability to overcome antifungal pine terpenoids and phenolics. Results We constructed seven cDNA libraries from eight G. clavigera isolates grown under various culture conditions, and Sanger sequenced the 5' and 3' ends of 25,000 cDNA clones, resulting in 44,288 high quality ESTs. The assembled dataset of unique transcripts (unigenes) consists of 6,265 contigs and 2,459 singletons that mapped to 6,467 locations on the G. clavigera reference genome, representing ~70% of the predicted G. clavigera genes. Although only 54% of the unigenes matched characterized proteins at the NCBI database, this dataset extensively covers major metabolic pathways, cellular processes, and genes necessary for response to environmental stimuli and genetic information processing. Furthermore, we identified genes expressed in spores prior to germination, and genes involved in response to treatment with lodgepole pine phloem extract (LPPE). Conclusions We provide a comprehensively annotated EST dataset for G. clavigera that represents a rich resource for gene characterization in this and other ophiostomatoid fungi. Genes expressed in response to LPPE treatment are indicative of fungal oxidative stress response. We identified two clusters of potentially functionally related genes responsive to LPPE treatment. Furthermore, we report a simple method for identifying contig misassemblies in de novo assembled EST collections caused by gene overlap on the genome.
Date created
2010
DOI
doi:10.7939/R3599Z447
License information
Attribution 4.0 International
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Citation for previous publication
Hesse-Orce, U., Diguistini, S., Keeling, C.I., Wang, Y., Li, M., Henderson, H., Docking, R., Liao, N.Y., Robertson, G., Holt, R.A., Jones, S.J.M., Bohlmann, J., Breuil, C. (2010). Gene discovery for the bark beetle-vectored fungal tree pathogen Grosmannia clavigera. BMC Genomics, 11, 536. doi:10.1186/1471-2164-11-536.
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File title: Abstract
File title: Functional genomics of mountain pine beetle (Dendroctonus ponderosae) midguts and fat bodies
File author: Tidiane Aw; Karen Schlauch; Christopher I Keeling; Sharon Young; Jeremy C Bearfield; Gary J Blomquist; Claus Tittiger
Page count: 12
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