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Permanent link (DOI): https://doi.org/10.7939/R3891245V

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Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinolenin Open Access

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Author or creator
Pan, Xue
Siloto, Rodrigo M. P.
Wickramarathna, Aruna D.
Mietkiewska, Elzbieta
Weselake, Randall J.
Additional contributors
Subject/Keyword
Plant Molecular Biology
Molecular Genetics
Phospholipid:diacylglycerol Acyltransferases
Flax
Metabolic Engineering
Triacylglycerol
α-Linolenic Acid
Lipid Metabolism
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently transfer ALA to TAG. To test this hypothesis, we conducted a systematic characterization of TAG-synthesizing enzymes from flax. We identified several genes encoding acyl-CoA:diacylglycerol acyltransferases (DGATs) and phospholipid:diacylglycerol acyltransferases (PDATs) from the flax genome database. Due to recent genome duplication, duplicated gene pairs have been identified for all genes except DGAT2-2. Analysis of gene expression indicated that two DGAT1, two DGAT2, and four PDAT genes were preferentially expressed in flax embryos. Yeast functional analysis showed that DGAT1, DGAT2, and two PDAT enzymes restored TAG synthesis when produced recombinantly in yeast H1246 strain. The activity of particular PDAT enzymes (LuPDAT1 and LuPDAT2) was stimulated by the presence of ALA. Further seed-specific expression of flax genes in Arabidopsis thaliana indicated that DGAT1, PDAT1, and PDAT2 had significant effects on seed oil phenotype. Overall, this study indicated the existence of unique PDAT enzymes from flax that are able to preferentially catalyze the synthesis of TAG containing ALA acyl moieties. The identified LuPDATs may have practical applications for increasing the accumulation of ALA and other polyunsaturated fatty acids in oilseeds for food and industrial applications.
Date created
2013
DOI
doi:10.7939/R3891245V
License information
© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
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Citation for previous publication
Pan, X., Siloto, R. M. P., Wickramarathna, A. D., Mietkiewska, E., & Weselake, R. J. (2013). Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinolenin. Journal of Biological Chemistry, 288(33), 24173-24188.  http://dx.doi.org/10.1074/jbc.M113.475699

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