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Permanent link (DOI): https://doi.org/10.7939/R31C1TV5W

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MicroRNAs in bovine adipogenesis: Genomic context, expression and function Open Access

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Author or creator
Romao, Josue M.
Jin, Weiwu
He, Maolong
McAllister, Tim
Guan, Leluo
Additional contributors
Subject/Keyword
Cluster
Fat Metabolism
Adipose Tissue
Genomic Context
Adipogenesis
Bovine
MicroRNA
Co-Expression
Species Specific
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Background MicroRNAs (miRNAs) are small non-coding RNAs found to regulate several biological processes including adipogenesis. Understanding adipose tissue regulation is critical for beef cattle as fat is an important determinant of beef quality and nutrient value. This study analyzed the association between genomic context characteristics of miRNAs with their expression and function in bovine adipose tissue. Twenty-four subcutaneous adipose tissue biopsies were obtained from eight British-continental crossbred steers at 3 different time points. Total RNA was extracted and miRNAs were profiled using a miRNA microarray with expression further validated by qRT-PCR. Results A total of 224 miRNAs were detected of which 155 were expressed in all steers (n = 8), and defined as the core miRNAs of bovine subcutaneous adipose tissue. Core adipose miRNAs varied in terms of genomic location (59.5% intergenic, 38.7% intronic, 1.2% exonic, and 0.6% mirtron), organization (55.5% non-clustered and 44.5% clustered), and conservation (49% highly conserved, 14% conserved and 37% poorly conserved). Clustered miRNAs and highly conserved miRNAs were more highly expressed (p 
Date created
2014
DOI
doi:10.7939/R31C1TV5W
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Attribution 4.0 International
Citation for previous publication
Romao, J. M., Jin, W., He, M., McAllister, T., & Guan, L. L. (2014). MicroRNAs in bovine adipogenesis: Genomic context, expression and function. BMC Genomics, 15(137), [15 pages].  http://dx.doi.org/10.1186/1471-2164-15-137

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Copyright note: � 2014 Romao et al.; licensee BioMed Central Ltd.
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