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Picodiscs for facile protein-glycolipid interaction analysis Open Access

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Author or creator
Leney, Aneika C.
Darestani, Reza Rezaei
Li, Jun
Nikjah, Sanaz
Kitova, Elena N.
Zou, Chunxia
Cairo, Christopher W.Xiong, Zi Jian
Privé, Gilbert G.
Klassen, John S.
Additional contributors
Subject/Keyword
Ligand
Substrate recognition
Receptor
Cholera-toxin binding
Mammalian sialidases
Microarrays
GM1 ganglioside
Ionization mass-spectrometry
Phospholipid-bilayer nanodiscs
Membrane-proteins
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Protein interactions with glycolipids are implicated in diverse cellular processes. However, the study of protein–glycolipid complexes remains a significant experimental challenge. Here, we describe a powerful new assay that combines electrospray ionization mass spectrometry (ESI-MS) and picodiscs, which are composed of human sphingolipid activator protein saposin A and a small number of phospholipids, to display glycolipids in a lipid environment for protein–glycolipid interaction studies in aqueous solution. Time-resolved measurements of enzyme catalyzed hydrolysis of glycolipid substrates and the detection of low, moderate, and high affinity protein–glycolipid interactions serve to demonstrate the reliability and versatility of the assay.
Date created
2015
DOI
doi:10.7939/R39S1M01W
License information
© 2015 Leney, A. C., Darestani, R. R., Li, J., Nikjah, S., Kitova, E. N., Zou, C., Cairo, C. W., Xiong, Z. J., Prive, G. G., & Klassen, J. S. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
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Citation for previous publication
Leney, A. C., Darestani, R. R., Li, J., Nikjah, S., Kitova, E. N., Zou, C., Cairo, C. W., Xiong, Z. J., Prive, G. G., & Klassen, J. S. (2015). Picodiscs for facile protein-glycolipid interaction analysis. Analytical Chemistry, 87(8), 4402-4408.  http://doi.org/10.1021/acs.analchem.5b00170

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