ERA

Download the full-sized PDF of Microbial lipid production using aqueous by-product stream from a lipid pyrolysis technologyDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R37D2QN23

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Microbial lipid production using aqueous by-product stream from a lipid pyrolysis technology Open Access

Descriptions

Other title
Subject/Keyword
aqueous by-product streams
biosolids utilization
microbial lipid production
algae lipids
yeast lipids
volatile fatty acids
waste feedstocks
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Mameeva, Olga G
Supervisor and department
Bressler, David (Agricultural, Food, and Nutritional Science)
Examining committee member and department
Bressler, David (Agricultural, Food, and Nutritional Science)
Ganzle, Michael (Agricultural, Food, and Nutritional Science)
Stuart, David (Biochemistry Science)
Case, Rebecca (Biological Sciences)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization
Bioresource and Food Engineering
Date accepted
2017-08-28T13:55:13Z
Graduation date
2017-11:Fall 2017
Degree
Master of Science
Degree level
Master's
Abstract
Currently, non-food carbon sources are making a valuable contribution to development of the next generation of hydrocarbon-based renewable fuels and value-added products. As non-food carbon feedstocks with low or negative value, yellow and brown greases, as well as biosolids, can potentially be processed through pyrolysis technologies to produce renewable fuels. Aqueous by-product streams are generated in these processes after hydrolysis, as well as after pyrolysis, and can potentially be used as a carbon and nutrient source to grow oleaginous microorganisms and to facilitate lipid production. In this study, volatile fatty acids and other compounds were quantified in pyrolysis aqueous by-product streams. It was shown that volatile fatty acids, such as acetic, propionic and butyric acids, could be used as single or mixed carbon sources for fermentation of Cryptococcus curvatus and Chlorella protothecoides. Model media mimicked the volatile fatty acid levels in the original aqueous pyrolysis by-product stream, which needed to be diluted to enable growth and lipid accumulation in microbial cells. To avoid potential inhibition caused by low pH, neutralization of the original waste streams and model media was performed to allow high biomass and lipid production. The best growth and lipid productivity of C. curvatus along with C. protothecoides biomass were observed in three-times diluted model media. It was shown that volatile fatty acid consumption by C. curvatus and C. protothecoides is time- and concentration-dependent. Moreover, the lipid production of C. curvatus and C. protothecoides using acetic acid, propionic acid, and butyric acid as a single and mixed carbon source were comparable, in some cases, with glucose. The fatty acid profiles of C. curvatus grown in the volatile fatty acids in the model media showed a higher amount of unsaturation in comparison to C. protothecoides fatty acid profiles. The aqueous by-product streams after hydrolysis of 4 % biosolids, brown grease, and their mixtures, were characterized for their ability to promote C. curvatus and C. protothecoides growth and lipid accumulation. It was established that the aqueous by-product streams after hydrolysis of 4 % biosolids or a biosolids/brown grease mix cannot be used for fermentation and lipid production by oleaginous microorganisms unless supplemented with the essential minerals to stimulate oleaginous microorganisms growth as well as glycerol to increase the carbon to nitrogen ratio to force lipid production, respectively. In supplemented conditions, a significant rise in biomass production was detected compared to low amounts of total lipids production for both C. curvatus and C. protothecoides. Moreover, substantial changes in fatty acids profiles of yeast and microalgae were detected. To summarize, the value-added application of the aqueous by-product streams after hydrolysis and pyrolysis of different lipid-containing feedstocks as a carbon and nutrient source to grow and produce lipids by oleaginous microorganisms is a promising approach. Both oleaginous microorganisms, C. curvatus and C. protothecoides, are suitable for high-concentration volatile fatty acids fermentations as well as for lipid production under the lipid-stimulated conditions.
Language
English
DOI
doi:10.7939/R37D2QN23
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2017-08-28T19:55:14.486+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (PDF/A)
Mime type: application/pdf
File size: 2962992
Last modified: 2017:11:08 16:43:46-07:00
Filename: Mameeva_Olga_G_201708_MSc.pdf
Original checksum: 1209cfb2d18fcdbc27f84e5ce3fa70e8
Activity of users you follow
User Activity Date